Modification of p115RhoGEF Ser(330) regulates its RhoGEF activity.
Modification of p115RhoGEF Ser(330) regulates its RhoGEF activity.
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DOI：
10.1016/j.cellsig.2013.06.012
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发表时间：
2013-11
影响因子：
4.8
通讯作者：
Kozasa T
中科院分区：
文献类型：
--
作者：
Chow CR;Suzuki N;Kawamura T;Hamakubo T;Kozasa T
p115RhoGEF is a member of a family of Rho-specific guanine nucleotide exchange factors that also contains a regulator of G protein signaling homology domain (RH-RhoGEFs) that serves as a link between Gα13 signaling and RhoA activation. While the mechanism of regulation of p115RhoGEF by Gα13 is becoming well-known, the role of other regulatory mechanisms, such as post-translational modification or autoinhibition, in mediating p115RhoGEF activity are less well-characterized. Here, putative phosphorylation sites on p115RhoGEF are identified and characterized. Mutation of Ser330 leads to a decrease in serum response element-mediated transcription as well as decreased activation by Gα13 in vitro. Additionally, this study provides the first report of the binding kinetics between full-length p115RhoGEF and RhoA in its various nucleotide states and examines the binding kinetics of phospho-mutant p115RhoGEF to RhoA. These data, together with other recent reports on regulatory mechanisms of p115RhoGEF, suggest that this putative phosphorylation site serves as a means for initiation or relief of autoinhibition of p115RhoGEF, providing further insight into the regulation of its activity.
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影响因子：
4.8
作者：
Bhattacharyya R;Banerjee J;Khalili K;Wedegaertner PB
通讯作者：
Wedegaertner PB
影响因子：
82.9
作者：
Guilluy, Christophe;Bregeon, Jeremy;Loirand, Gervaise
通讯作者：
Loirand, Gervaise
影响因子：
3.5
作者：
Bhattacharyya, R;Wedegaertner, PB
通讯作者：
Wedegaertner, PB
DOI：
10.1074/jbc.m110.154732
发表时间：
2011-01-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Daigo K;Kawamura T;Ohta Y;Ohashi R;Katayose S;Tanaka T;Aburatani H;Naito M;Kodama T;Ihara S;Hamakubo T
通讯作者：
Hamakubo T
影响因子：
4.8
作者：
Kreutz, Barry;Hajicek, Nicole;Kozasa, Tohru
通讯作者：
Kozasa, Tohru