THE ALPHA-TUBULINS AND BETA-TUBULINS OF TOXOPLASMA-GONDII ARE ENCODED BY SINGLE COPY GENES CONTAINING MULTIPLE INTRONS
THE ALPHA-TUBULINS AND BETA-TUBULINS OF TOXOPLASMA-GONDII ARE ENCODED BY SINGLE COPY GENES CONTAINING MULTIPLE INTRONS
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DOI：
10.1016/0166-6851(88)90081-3
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发表时间：
1988-06-01
影响因子：
1.5
通讯作者：
BOOTHROYD, JC
中科院分区：
文献类型：
--
作者：
NAGEL, SD;BOOTHROYD, JC
我们通过详细分析编码α的基因，研究了弓形虫原虫基因表达的基本原理。-和-微管蛋白。南方分析和定量研究表明，与迄今为止研究的其他生物体不同，这两种基因在单倍体弓形虫基因组中以单拷贝形式存在。这些基因的测序表明它们都含有多个保守的5‘和3’剪接的内含子。站点的信号。我们已经发现，HeLa细胞核提取物能够准确地拼接弓形虫前mrna结构。我们已经映射了。引物延伸和RNase保护实验确定了-微管蛋白基因转录起始的确切位点和聚A添加的大致位点。反式剪接，如在着丝质体中所示，不参与成熟α的形成。刚地弓形虫-微管蛋白转录本。
We have studied the fundamentals of gene expression in the protozoan parasite Toxoplasma gondii by analyzing, in detail, the genes encoding .alpha.- and .beta.-tubulin. Southern analysis and quantitation studies reveal that, unlike in other organisms studied thus far, both these genes are present as single copies in the haploid Toxoplasma genome. Sequencing of these genes indicates that they both contain multiple introns with conserved 5'' and 3'' splice. site signals. We have found that HeLa cell nuclear extracts are able to accurately splice a Toxoplasma pre-mRNA construct. We have mapped, for the .alpha.-tubulin gene, the exact site of transcription initiation and the approximate site of poly A addition by primer extension and RNase protection assays. Trans-splicing, as demonstrated in the Kinetoplastida, is not involved in the formation of the mature .alpha.-tubulin transcript in T. gondii.