Progressive ordering with decreasing temperature of the phospholipids of influenza virus
Progressive ordering with decreasing temperature of the phospholipids of influenza virus
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DOI：
10.1038/nchembio.77
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发表时间：
2008-04-01
影响因子：
14.8
通讯作者：
Zimmerberg, Joshua
中科院分区：
文献类型：
--
作者：
Polozov, Ivan V.;Bezrukov, Ludmila;Zimmerberg, Joshua
利用质子魔角自旋核磁共振谱中脂烃链共振的线宽和自旋边带强度，我们检测了无外源探针的完整流感病毒天然存在的脂质的温度依赖性相态。越来越多地，低于41摄氏度的有序和无序的脂质结构域共存的病毒包膜及其提取物。在22 ℃时，许多脂质处于凝胶相，其分数随着胆固醇消耗而可逆地增加。扩散测量和荧光显微镜独立确认凝胶相域的存在。因此，现在证明了生物膜中脂质有序区域的存在。以上的流感感染的生理温度，病毒包膜脂质的物理性质，无论蛋白质含量，是无法区分的无序部分。病毒融合似乎是不相关的有序脂质含量。脂质排序可能有助于病毒在较低温度下的稳定性，最近发现这对空气传播至关重要。
Using linewidth and spinning sideband intensities of lipid hydrocarbon chain resonances in proton magic angle spinning NMR spectra, we detected the temperature-dependent phase state of naturally occurring lipids of intact influenza virus without exogenous probes. Increasingly, below 41 degrees C ordered and disordered lipid domains coexisted for the viral envelope and extracts thereof. At 22 degrees C much lipid was in a gel phase, the fraction of which reversibly increased with cholesterol depletion. Diffusion measurements and fluorescence microscopy independently confirmed the existence of gel-phase domains. Thus the existence of ordered regions of lipids in biological membranes is now demonstrated. Above the physiological temperatures of influenza infection, the physical properties of viral envelope lipids, regardless of protein content, were indistinguishable from those of the disordered fraction. Viral fusion appears to be uncorrelated to ordered lipid content. Lipid ordering may contribute to viral stability at lower temperatures, which has recently been found to be critical for airborne transmission.