Effects of the pineal hormone melatonin on the anchorage-independent growth of human breast cancer cells (MCF-7) in a clonogenic culture system.
Effects of the pineal hormone melatonin on the anchorage-independent growth of human breast cancer cells (MCF-7) in a clonogenic culture system.
复制标题
松果体激素褪黑激素对克隆培养系统中人乳腺癌细胞 (MCF-7) 贴壁独立生长的影响。
DOI：
10.1016/0304-3835(90)90240-x
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发表时间：
1990
期刊：
影响因子：
9.7
通讯作者：
Blask,DE
中科院分区：
文献类型：
--
作者：
Cos,S;Blask,DE
只有生理水平的褪黑素对生长在锚定依赖培养系统中的MCF-7乳腺癌细胞具有抗增殖作用。我们研究了褪黑素对MCF-7细胞非贴壁生长的影响，以及在克隆培养条件下这种吲哚胺的剂量-反应特性。褪黑素的抑制作用与形成的菌落数目和大小有关，呈线性剂量-反应曲线，药物浓度的抑制作用最大，亚生理水平的褪黑素抑制作用最小。这些结果表明，细胞附着可能改变MCF-7细胞对褪黑素的敏感性。
Only physiological levels of melatonin exert an antiproliferative effect on MCF-7 breast cancer cells grown in an anchorage-dependent culture system. We investigated melatonin's effect on the anchorage-independent growth of MCF-7 cells as well as the dose-response characteristics of this indoleamine under clonogenic culture conditions. Melatonin's inhibitory effect, with respect to the number and size of colonies formed, exhibit a linear dose-response curve with pharmacological concentrations producing a maximal inhibition while subphysiological levels of melatonin induce minimal inhibition. These results indicate that cellular attachment may modify the sensitivity of MCF-7 cells towards melatonin.