Localization of neurons in the rat spinal cord which project to the superior cervical ganglion.
Localization of neurons in the rat spinal cord which project to the superior cervical ganglion.
复制标题
大鼠脊髓中投射到颈上神经节的神经元的定位。
DOI：
10.1002/cne.901960107
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Zigmond,RE
中科院分区：
文献类型：
--
作者：
Rando,TA;Bowers,CW;Zigmond,RE
用辣根过氧化物酶(HRP)法对大鼠脊髓内投射到颈上神经节的神经元进行定位。将HRP应用于颈交感神经干近端切开，靠近其进入颈上神经节。存活3天、6天或9天后，处死动物，用二氨基联苯胺、联苯胺二盐酸盐或四甲基联苯胺处理脊髓以显示HRP。标记神经元仅位于HRP应用部位的同侧，并局限于脊髓C8-T5节段。其中90%的神经元位于T1-T3节段。存活时间为3天和6天的11个实验的平均标记神经元数为1575±89个。应用HRP后第9天，平均标记细胞数和标记细胞密度均减少。标记神经元分布于脊髓的四个区域：中间外侧核(75%)、外侧索(23%)、中央自主神经区(1%)和间隔区(1%)。中间外侧核的细胞并不是沿着脊髓的肩尾轴形成连续的柱状，而是多呈簇状分布，每个脊髓节段都有几个簇状细胞。
Horseradish peroxidase (HRP) was used to determine the location in the spinal cord of neurons projecting to the superior cervical ganglion of the rat. HRP was applied to the proximal cut end of the cervical sympathetic trunk, close to its entry into the superior cervical ganglion. After survival times of 3, 6, or 9 days, the animals were sacrificed and their spinal cords were processed to visualize HRP using diaminobenzidine, benzidine dihydrochloride, or tetramethylbenzidine. Labeled neurons were found only ipsilateral to the site of HRP application and were restricted to spinal segments C8‐T5. Ninety percent of these neurons were located in segments T1‐T3. Similar numbers of labeled neurons were found at survival times of 3 and 6 days and the mean number ± S.E.M. for 11 experiments at these two survival times was 1575 ± 89. Nine days after application of HRP the mean number of labeled cells and the density of label per cell were reduced. Labeled neurons were found in four regions of the spinal cord: the intermediolateral nucleus (75%), the lateral funiculus (23%), the central autonomic area (1%), and the intercalated region (1%). The cells of the intermediolateral nucleus did not form a continuous column along the rostrocaudal axis of the spinal cord, but instead were often found in clusters, several clusters being present per spinal segment.