Interaction between linc01615 and miR-491-5p regulates the survival and metastasis of colorectal cancer cells.
Interaction between linc01615 and miR-491-5p regulates the survival and metastasis of colorectal cancer cells.
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linc01615与miR-491-5p的相互作用调节结直肠癌细胞的存活和转移
DOI：
10.21037/tcr.2020.03.03
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发表时间：
2020-04
影响因子：
0.9
通讯作者：
Xie B
中科院分区：
文献类型：
--
作者：
Xiao Y;Hu F;Li M;Mo L;Xu C;Wang X;Nie J;Yang L;Xie B
Background The 5-year survival of colorectal cancer (CRC) has had no obvious improvement during the past decades, although a significant progress in treatments has been established. The molecular mechanisms that drive the progression of CRC are still unclear. This study aims to determine the biological activities of linc01615 and its regulatory microRNAs in CRC cells. Methods The expression of linc016150 and miR-491-5p was measured in six CRC cell lines and one normal colon mucosal epithelial cell line. Their effects on cell proliferation, apoptosis, invasion, and migration were tested in Caco-2 cells. Results Linc01615 mRNA expression was upregulated in six colorectal cancer cell lines compared to the normal colon mucosal epithelial cell line, which was negatively regulated by miR-491-5p in Caco-2 cells. Silencing of linc01615 gene expression significantly decreased cell proliferation, increased apoptosis, and inhibited invasion and migration in Caco-2 cells. Overexpression of linc01615 exhibited an opposite effect on silencing of linc01615 expression. Transfection with miR-491-5p mimics downregulated linc01615 expression and inhibited the biological activities of linc01615. In contrast, the inhibitor of miR-491-5p up-regulated linc01615 expression and subsequently enhanced the biological activities of linco1615. Also, overexpression of linc01615 can block the effects of miR-491-5p mimics in Caco-2 cells. Conclusions Linc01615 functions as an oncogene while miR-491-5p functions as a tumor suppressor in colorectal cancer cells through negatively regulating each other; both are involved in cell proliferation, apoptosis, invasion, and migration in colorectal cancer cells.
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DOI：
10.1038/nrm.2017.104
发表时间：
2018-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Ransohoff JD;Wei Y;Khavari PA
通讯作者：
Khavari PA
影响因子：
12.4
作者：
Yue, Ben;Cai, Donglan;Yan, Dongwang
通讯作者：
Yan, Dongwang
影响因子：
2.8
作者：
Zhou, Peng;Sun, Lixia;Sun, Lei
通讯作者：
Sun, Lei
影响因子：
3.8
作者：
Yu X;Mi L;Dong J;Zou J
通讯作者：
Zou J
影响因子：
2.7
作者：
Luo, Jihui;Guo, Yi;Zhou, Meihua
通讯作者：
Zhou, Meihua