Formation and dissolution of phospholipid domains with varying textures in hybrid lipo-polymersomes
Formation and dissolution of phospholipid domains with varying textures in hybrid lipo-polymersomes
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DOI：
10.1039/c2sm25646k
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发表时间：
2012-01-01
期刊：
影响因子：
3.4
通讯作者：
Beales, Paul A.
中科院分区：
文献类型：
--
作者：
Nam, Jin;Vanderlick, T. Kyle;Beales, Paul A.
设计具有可在广泛参数空间内调节化学和物理特性的新型软膜结构对于新型功能材料的开发非常重要。这些新型膜的许多潜在应用可能需要生物相容性界面，例如。例如，用于整合蛋白的功能重建。在这里，我们研究了通过将二嵌段共聚物聚（丁二烯-b-环氧乙烷）与常见的天然脂质（例如脂质）混合而产生的混合脂质聚合物囊泡（HLP）中成分异质性的形成和控制。例如，1，2-二棕榈酰-sn-甘油-3-磷酸胆碱或1，2-棕榈酰-油酰-sn-甘油-3-磷酸胆碱。富含脂质的结构域与混合囊泡的富含聚合物的基质的混合和分层是通过热驱动的相分离和胆固醇的包含来控制的。域的大小和形态可以分别通过冷却速率和脂质组成来控制。高分子添加剂，例如。例如，环糊精、酶和表面活性剂，可用于重塑杂化膜及其结构域，导致结构域溶解、内容物的受控释放或杂化囊泡的破裂。这些复合膜是基于封装技术的有前途的材料，这些技术需要生物相容性膜环境和增强的结构稳定性的结合，例如。例如，输送和传感应用或控制限制内的（生物）化学反应。
The design of novel, soft membrane structures with chemical and physical properties that are tunable across a broad spectrum of parameter space is important for the development of new functional materials. Many potential applications of these novel membranes will likely require biocompatible interfaces, e. g., for functional reconstitution of integral proteins. Here we investigate the formation and control of compositional heterogeneities in hybrid lipo-polymersomes (HLPs) created by mixing the diblock copolymer poly(butadiene-b-ethylene oxide) with common natural lipids, e. g., 1,2-dipalmitoyl-sn-glycero-3-phosphocholine or 1,2-palmitoyl-oleoyl-sn-glycero-3-phosphocholine. Mixing and demixing of lipid-rich domains from the polymer-rich matrix of hybrid vesicles is controlled via thermally driven phase separation and by the inclusion of cholesterol. Domain size and morphology can be controlled by cooling rate and lipid composition, respectively. Macromolecular additives, e. g., cyclodextrins, enzymes and surfactants, can be used to remodel the hybrid membrane and its domains, resulting in domain dissolution, controlled release of contents or rupture of the hybrid vesicles. These composite membranes are promising materials for encapsulation-based technologies that require the combination of biocompatible membrane environments and enhanced structural stability, e. g., delivery and sensing applications or controlling (bio)chemical reactions within confinement.