Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L.)
Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L.)
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DOI：
10.17221/67/2009-cjgpb
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发表时间：
2010-01-01
影响因子：
0.9
通讯作者：
Pakniyat, Hassan
中科院分区：
文献类型：
--
作者：
Abedi, Tayebeh;Pakniyat, Hassan
以10个甘蓝型油菜（Brassica napus L.）干旱胁迫条件下的品种。植物在温室中在三种灌溉制度（FC;田间容量、60%FC和30%FC）下生长。干旱胁迫优先提高超氧化物歧化酶（SOD）和愈创木酚过氧化物酶（POD）活性，降低过氧化氢酶（CAT）活性。据报道，在最佳和有限灌溉制度下酶活性水平最高的Licord是最耐受的品种。而Hyola 308和Oklahoma的酶活性最低，被认为是对干旱胁迫敏感的品种。非变性聚丙烯酰胺凝胶电泳（PAGE）分析了8种SOD同工酶。油菜叶片含有3种Mn-SOD同工酶和5种Cu/Zn-SOD同工酶。Mn-SOD的表达优先受干旱胁迫的影响。油菜叶片中检测到5种POD同工酶。干旱胁迫下POD-4和POD-5活性增强。结果表明，干旱胁迫条件下新同工酶谱带的出现可作为耐旱品种的生化标记。
The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.