Dielectrophoretic-Field Flow Fractionation Analysis of Dielectric, Density, and Deformability Characteristics of Cells and Particles
Dielectrophoretic-Field Flow Fractionation Analysis of Dielectric, Density, and Deformability Characteristics of Cells and Particles
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DOI：
10.1021/ac901470z
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发表时间：
2009-11-01
影响因子：
7.4
通讯作者：
Gascoyne, Peter R. C.
中科院分区：
文献类型：
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作者：
Gascoyne, Peter R. C.
介电电泳场流分离（DEP-FFF）已被用于区分颗粒和细胞的基础上，他们的介电和密度特性。然而，流体动力升力（HDLF）在快速分离所需的流速没有占在以前的理论治疗的方法。此外，没有开发出直接从DEP-FFF洗脱数据中分离颗粒或细胞物理特性的方法。DEP-FFF的一个扩展的理论，帐户HDLF。与使用DS 19 etythroleukemia细胞作为模型粒子与频率依赖性的介电特性，它表明，修订后的理论占DEP-FFF洗脱行为在很宽的范围内的条件下，是一致的沉降-FFF时，DEP力为零。在特定条件下进行四次洗脱运行，该理论允许推导细胞密度分布，并提供了良好的估计分布的介电性能的细胞和它们的变形特性，影响HDLF。该方法允许快速分析细胞的生物物理特性，鉴定和表征亚群，以及设计最佳DEP-FFF分离条件。扩展后的DEP-FFF理论具有广泛的适用性，其参数测量方法也可适用于其他类型的粒子。
Dielectrophoretic field-flow fractionation (DEP-FFF) has been used to discriminate between particles and cells based on their dielectric and density properties. However, hydrodynamic lift forces (HDLF) at flow rates needed for rapid separations were not accounted for in the previous theoretical treatment of the approach. Furthermore, no method was developed to isolate particle or cell physical characteristics directly from DEP-FFF elution data. An extended theory of DEP-FFF is presented that accounts for HDLF. With the use of DS19 etythroleukemia cells as model particles with frequency-dependent dielectric properties, it is shown that the revised theory accounts for DEP-FFF elution behavior over a wide range of conditions and is consistent with sedimentation-FFF when the DEP force is zero. Conducting four elution runs under specified conditions, the theory allows for the derivation of the cell density distribution and provides good estimates of the distributions of the dielectric properties of the cells and their deformability characteristics that affect HDLF. The approach allows for rapid profiling of the biophysical properties of cells, the identification and characterization of subpopulations, and the design of optimal DEP-FFF separation conditions. The extended DEP-FFF theory is widely applicable, and the parameter measurement methods may be adapted easily to other types of particles.