Alternative Oxidase in Resistance to Biotic Stresses: Nicotiana attenuata AOX Contributes to Resistance to a Pathogen and a Piercing-Sucking Insect But Not Manduca sexta Larvae1[W][OA]
Alternative Oxidase in Resistance to Biotic Stresses: Nicotiana attenuata AOX Contributes to Resistance to a Pathogen and a Piercing-Sucking Insect But Not Manduca sexta Larvae1[W][OA]
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DOI：
10.1104/pp.112.200865
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发表时间：
2012-09
期刊：
影响因子：
7.4
通讯作者：
Lu Zhang;Youngjoo Oh;Hongyu Li;I. Baldwin;I. Galis
中科院分区：
文献类型：
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作者：
Lu Zhang;Youngjoo Oh;Hongyu Li;I. Baldwin;I. Galis
在替代氧化酶（AOX）基因表达沉默的转基因烟草（Nicotiana attenuata）植物（irAOX）中检查了替代呼吸途径在保护植物免受生物胁迫中的作用。野生型和 irAOX 植物分别受到 (1) 咀嚼食草动物 (Manduca sexta)、(2) 刺吸式昆虫 (Empoasca spp.) 和 (3) 细菌病原体 (Pseudomonas syringae pv tomato DC3000) 的攻击，表明所有这些处理都能强烈引发野生型植物中 AOX 基因转录物的积累。当检查 N. attenuata 化学防御和抗性时，irAOX 植物表现出野生型水平的防御相关植物激素、次级代谢产物和对 M. sexta 的抗性。相比之下，与田间和/或温室中的野生型植物相比，刺吸式叶蝉 (Empoasca spp.) 造成更多的叶片损伤，并导致 irAOX 中的水杨酸水平显着升高。随后，与野生型植物相比，在小绿叶蝉属的长期攻击下，irAOX植物积累了较低水平的防御代谢物、17-羟基香叶醛二萜糖苷、咖啡酰腐胺和尼古丁。在温室里。最后，在感染丁香假单胞菌的 irAOX 植物中观察到加速的细胞死亡表型，这与与野生型叶子相比，病原体感染的 irAOX 叶子中更高水平的水杨酸和过氧化氢水平相关。总体而言，与 AOX 相关的植物激素和/或氧化还原水平的变化似乎支持减毒烟草植物对细胞刺吸式昆虫的抵抗力，并调节病原体感染组织中细胞死亡的进程，但对快速进食的专食草食动物 M. sexta 无效。
The role of the alternative respiratory pathway in the protection of plants against biotic stress was examined in transgenic tobacco (Nicotiana attenuata) plants (irAOX) silenced in the expression of ALTERNATIVE OXIDASE (AOX) gene. Wild-type and irAOX plants were independently challenged with (1) chewing herbivores (Manduca sexta), (2) piercing-sucking insects (Empoasca spp.), and (3) bacterial pathogens (Pseudomonas syringae pv tomato DC3000), showing that all these treatments can strongly elicit accumulation of AOX gene transcripts in wild-type plants. When N. attenuata chemical defenses and resistance were examined, irAOX plants showed wild-type levels of defense-related phytohormones, secondary metabolites, and resistance to M. sexta. In contrast, piercing-sucking leafhoppers (Empoasca spp.) caused more leaf damage and induced significantly higher salicylic acid levels in irAOX compared with wild-type plants in the field and/or glasshouse. Subsequently, irAOX plants accumulated lower levels of defense metabolites, 17-hydroxygeranyllinalool diterpene glycosides, caffeoylputrescine, and nicotine compared with wild-type plants under prolonged attack of Empoasca spp. in the glasshouse. Finally, an accelerated cell death phenotype was observed in irAOX plants infected with P. syringae, which correlated with higher levels of salicylic acid and hydrogen peroxide levels in pathogen-infected irAOX compared with wild-type leaves. Overall, the AOX-associated changes in phytohormone and/or redox levels appear to support the resistance of N. attenuata plants against cell piercing-sucking insects and modulate the progression of cell death in pathogen-infected tissues but are not effective against rapidly feeding specialist herbivore M. sexta.