Turn-on near-infrared electrochemiluminescence sensing of thrombin based on resonance energy transfer between CdTe/CdS core(small)/shell(thick) quantum dots and gold nanorods
Turn-on near-infrared electrochemiluminescence sensing of thrombin based on resonance energy transfer between CdTe/CdS core(small)/shell(thick) quantum dots and gold nanorods
复制标题
基于 CdTe/CdS 核（小）/壳（厚）量子点与金纳米棒之间共振能量转移的凝血酶开启近红外电化学发光传感
DOI：
10.1016/j.bios.2016.03.057
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发表时间：
2016
影响因子：
12.6
通讯作者：
Han Heyou
中科院分区：
文献类型：
--
作者：
Wang Jing;Jiang Xiaochun;Han Heyou
本文设计了一种近红外电化学发光（NECL）适体传感器，用于凝血酶的超灵敏测定。它是基于CdTe/CdS核/壳厚量子点（QDs）与金纳米棒（AuNRs）的ECL共振能量转移（ECL-RET）。通过核酸适体与互补寡核苷酸的DNA杂交，将具有ECL受体功能的金核受体组装到CdTe/CdS膜上。在没有凝血酶的情况下，由于量子点与金核受体的ECL-RET，量子点的NECL被淬灭。在凝血酶的存在下，由于特异性的适配体-蛋白质亲和相互作用，凝血酶可以取代量子点膜上的AuNR，因此系统的NECL被“打开”。在100 aM ~ 10 fM范围内，电化学发光强度增量与凝血酶浓度呈对数线性关系，检出限为31 aM（S/N=3）。重要的是，开发的适体传感器成功地应用于真实的血清样品中的凝血酶传感。
Here we designed a near-infrared electrochemiluminescence (NECL) aptasensor for turn-on ultrasensitive determination of thrombin. It was based on the ECL resonance energy transfer (ECL-RET) of CdTe/CdS coresmall/shellthickquantum dots (QDs) to gold nanorods (AuNRs). AuNRs which functioned as ECL acceptors were assembled onto CdTe/CdS film by DNA hybridization between aptamers and their complementary oligonucleotides. In the absence of thrombin, the NECL of QDs was quenched as a result of the ECL-RET of QDs to AuNRs. In the presence of thrombin, the NECL of the system was “turned on” because thrombin can replace the AuNRs onto the QDs film, owing to the specific aptamer-protein affinity interactions. In this way, the increment of ECL intensity and the concentration of thrombin showed a logarithmic linear correlation in the range of 100 aM to 10 fM with a detection limit of 31 aM (S/N=3). Importantly, the developed aptasensor was successfully applied to thrombin sensing in real serum samples.