The feeding of beta-carotene down-regulates serum IgE levels and inhibits the type I allergic response in mice.
The feeding of beta-carotene down-regulates serum IgE levels and inhibits the type I allergic response in mice.
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喂食 β-胡萝卜素可下调小鼠血清 IgE 水平并抑制 I 型过敏反应。
DOI：
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发表时间：
2004
影响因子：
2
通讯作者：
T. Maitani
中科院分区：
文献类型：
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作者：
Yuji Sato;H. Akiyama;H. Suganuma;Takahiro Watanabe;M. Nagaoka;T. Inakuma;Y. Goda;T. Maitani
将含有β-胡萝卜素的饲料经口给予用卵清蛋白（OVA）腹腔内免疫的BALB/c小鼠约1个月。测定小鼠血清中OVA特异性IgE、OVA特异性IgG 1和OVA特异性IgG 2a的滴度。β-胡萝卜素对小鼠卵清蛋白特异性IgE效价和卵清蛋白特异性IgG 1效价有显著抑制作用。另一方面，在喂食β-胡萝卜素的小鼠中，OVA特异性IgG 2a滴度显著高于对照小鼠。β-胡萝卜素喂养的OVA特异性IgE抑制是剂量依赖性的。我们还研究了进食β-胡萝卜素对主动全身过敏反应的影响。用卵清蛋白免疫的小鼠饲喂β-胡萝卜素可抑制抗原刺激引起的体温立即降低。此外，在主动全身过敏反应下，喂食β-胡萝卜素的小鼠血清组胺的增加低于对照组。然后，我们研究了小鼠脾细胞产生细胞因子的模式，然后在体外用OVA再刺激。来自喂食β-胡萝卜素的小鼠的脾细胞比来自对照组的脾细胞产生更多的IFN-γ、IL-12和IL-2。相反，来自喂食β-胡萝卜素的小鼠的脾细胞产生的IL-4、IL-5、IL-6、IL-10比来自对照组的那些少。此外，使用实时定量RT-PCR技术对脾细胞的IFN-γ mRNA水平的分析揭示了来自喂食β-胡萝卜素的小鼠的脾细胞中的更高水平。这些发现表明，喂养β-胡萝卜素改善辅助T细胞（T（H））1-T（H）2平衡，抑制特异性IgE和IgG 1的产生和抗原诱导的过敏反应。
Feed containing beta-carotene was administered orally to BALB/c mice immunized intraperitoneally with ovalbumin (OVA) for approximately 1 month. The titers of OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a in the mouse sera were determined. The OVA-specific IgE titer and OVA-specific IgG1 titer by mice fed beta-carotene were significantly inhibited. On the other hand, the OVA-specific IgG2a titer in mice fed beta-carotene was significantly greater than those of control mice. The OVA-specific IgE suppression of beta-carotene feeding was dose-dependent. We also examined the effect of fed beta-carotene on active systemic anaphylaxis. Feeding beta-carotene to mice immunized with OVA inhibited the immediate reduction of the body temperature induced by antigen stimulation. Furthermore, the increase in serum histamine in the mice fed beta-carotene under active systemic anaphylaxis was lower than in controls. We then examined the pattern of cytokine production by spleen cells from mice followed by restimulation with OVA in vitro. The spleen cells from the mice fed beta-carotene produced more IFN-gamma, IL-12 and IL-2 than those from the control group. In contrast, the spleen cells from the mice fed beta-carotene produced less IL-4, IL-5, IL-6, IL-10 than those from the control group. Furthermore, analysis of IFN-gamma mRNA levels of the splenocytes using the real-time quantitative RT-PCR technique revealed higher levels in the splenocytes from the mice fed beta-carotene. These findings suggest that feeding beta-carotene improves the helper T cell (T(H))1-T(H)2 balance, inhibiting specific IgE and IgG1 production and antigen-induced anaphylactic response.