Black-pigmented anaerobic rods in closed periapical lesions
Black-pigmented anaerobic rods in closed periapical lesions
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DOI：
10.1046/j.1365-2591.1999.00216.x
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发表时间：
1999-05-01
影响因子：
5
通讯作者：
Slots, J
中科院分区：
文献类型：
--
作者：
Bogen, G;Slots, J
目的本研究确定了20个封闭的根尖周病变与症状和无症状的难治性endodonticdiseases.Methodology相关的牙髓卟啉单胞菌，牙龈卟啉单胞菌，中间普雷沃菌和变黑普雷沃菌的频率要deliniate可能的口腔来源的牙髓卟啉单胞菌，存在的有机体进行了评估，在选定的龈下部位和唾液在同一研究患者。根尖周样本是在外科牙髓手术过程中通过纸尖获得的，使用的方法旨在最大限度地减少非牙髓微生物的污染。龈下菌斑样本通过纸尖从三个牙周袋和与封闭的根尖周病变相关的牙齿袋中获得。从软腭表面收集未受刺激的唾液。细菌鉴定进行了使用种特异性聚合酶链反应（PCR）检测方法。结果P。牙髓没有确定在任何根尖周病变，即使从8例（40%）的龈下样本显示P，牙髓特异性扩增子。牙龈卟啉单胞菌发生在一个根尖周病变，与中度疼痛。P. nigrescens，P. Endodontalis和P. intermedia没有检测到任何根尖周病变studyed.Conclusions黑色色素厌氧杆似乎是罕见的居民的封闭根尖周病变。
Aim This study determined the frequency of Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens in 20 closed periapical lesions associated with symptomatic and asymptomatic refractory endodontic disease.Methodology To deliniate possible oral sources of P. endodontalis, the presence of the organism was assessed in selected subgingival sites and saliva in the same study patients. Periapical samples were obtained by paper points during surgical endodontic procedures using methods designed to minimize contamination by non-endodontic microorganisms. Subgingival plaque samples were obtained by paper points from three periodontal pockets and from the pocket of the tooth associated with the closed periapical lesion. Unstimulated saliva was collected from the surface of the soft palate. Bacterial identification was performed using a species-specific polymerase chain reaction (PCR) detection method.Results P. Endodontalis was not identified in any periapical lesion, even though subgingival samples from eight patients (40 %) revealed the P, endodontalis-specific amplicon. P. gingivalis occurred in one periapical lesion that was associated with moderate pain. P. nigrescens, P. Endodontalis and P. intermedia were not detected in any periapical lesion studied.Conclusions Black-pigmented anaerobic rods appear to be infrequent inhabitants of the closed periapical lesion.