Long-term immunity and protection against herpes simplex virus type 2 in the murine female genital tract after mucosal but not systemic immunization
Long-term immunity and protection against herpes simplex virus type 2 in the murine female genital tract after mucosal but not systemic immunization
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DOI：
10.1086/515286
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发表时间：
1998-05-01
影响因子：
6.4
通讯作者：
Rosenthal, KL
中科院分区：
文献类型：
--
作者：
Gallichan, WS;Rosenthal, KL
用表达单纯疱疹病毒糖蛋白B(HSV-2)的重组腺病毒载体(AdgB8)经鼻腔(Inl)或腹腔(IP)免疫小鼠，观察其对女性生殖道HSV-2感染的免疫程度和免疫持续时间。经阴道感染HSV-2后，对照组小鼠迅速发病，阴道冲洗液中病毒滴度较高。相反，在INL和IP免疫的小鼠中，病毒滴度显著下降，且下降的速度相似，到第7天时，在阴道冲洗样本中检测不到病毒。对生殖器病理和存活率的评估表明，只有免疫接种才能提供长期保护。阴道病毒滴度下降时对抗体分泌细胞(ASCs)的检测表明，仅在Inl免疫的小鼠生殖器组织中观察到GB特异性的IgA ASCs。这些结果表明，粘膜免疫提供了对女性生殖道性传播病毒感染的高而持久的免疫力。
The degree and duration of immunity against herpes simplex virus type 2 (HSV-2) infection of the female genital tract were assessed after intranasal (inl) or intraperitoneal (ip) immunization with a recombinant adenovirus vector expressing HSV glycoprotein B (AdgB8). After intravaginal HSV-2 challenge, control mice rapidly developed disease and displayed high virus titers in vaginal washes. In contrast, virus titers decreased significantly and at similar rates in inl and ip immunized mice and by day 7 were undetectable in vaginal wash samples. Assessment of genital pathology and survival showed that only inl immunization provided long-term protection. Examination of antibody-secreting cells (ASCs) during the decline in vaginal virus titers revealed that gB-specific IgA ASCs were only observed in the genital tissues of inl immunized mice. These results indicate that mucosal immunization provides a high and long-lasting level of immunity from sexually transmitted viral infections of the female genital tract.