A facile fluorescent probe with a large Stokes shift for sequentially detecting copper and sulfide in 100% aqueous solution and imaging them in living cells
A facile fluorescent probe with a large Stokes shift for sequentially detecting copper and sulfide in 100% aqueous solution and imaging them in living cells
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A%20facile%20荧光%20probe%20with%20a%20large%20Stokes%20shift%20for%20sequentially%20检测%20copper%20and%20sulfide%20in%20100%%20aqueous%20solution%20and%20imaging%20them%20in%20living%20cells
DOI：
10.1016/j.snb.2017.10.124
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发表时间：
2018-03-01
影响因子：
8.4
通讯作者：
Guo, Yuan
中科院分区：
文献类型：
--
作者：
Wang, Huan;Shi, Dong-Lei;Guo, Yuan
纯水系统中Cu2+和S2-的选择性检测是非常具有挑战性的。为了解决这一问题，我们提出了一种新型荧光探针CF536，用于在100%水溶液中通过基于荧光猝灭和ESIPT机制的位移法可逆检测Cu2+和S2-，并通过IR， NMR， HRMS和x射线分析进行了表征。在CF536的水溶液中加入1.0当量的Cu2+后，CF536的强绿色荧光在2 min内猝灭。在加入S2-（3.0当量）后，由于顺磁性Cu2+的消除，ESIPT探针的发射很快从CF536-Cu2+体系中恢复。通过交替添加Cu2+和S2-，荧光“ON-OFF-ON”循环可以重复至少5次，这表明CF536是一种可再生的双功能探针。该探针选择性高，响应时间快，由于引入了极亲水的三羟基而具有良好的水溶性，并且具有101 nm的大Stokes位移(lambda（abs) = 435 nm, lambda(em) = 536 nm）的抗干扰能力，因此该方法非常适合用于生物成像。CF536探针成功地用于活细胞中铜和硫化氢的荧光成像。(C) 2017 Elsevier B.V.版权所有
The selective detection of Cu2+ and S2- in pure water systems is very challenging. To address this problem, we here present a novel fluorescent probe CF536 for the reversible detection of Cu2+ and S2- in a 100% aqueous solution via a displacement approach based on fluorescence quenching and the ESIPT mechanism, and characterized by IR, NMR, HRMS and X-ray analysis. The introduction of Cu2+ (1.0 equiv.) to the aqueous solution of CF536 led to quenching of the intense green fluorescence within 2 min. On the addition of S2- (3.0 equiv.), the emission of the ESIPT probe soon recovered from the CF536-Cu2+ system due to the abolishment of paramagnetic Cu2+. The fluorescence "ON-OFF-ON" cycle can be repeated a minimum of 5 times by the alternate addition of Cu2+ and S2-, implying that CF536 is a renewable dual-functional probe. The probe displayed high selectivity, a rapid response time, excellent water solubility due to introduction of the extremely hydrophilic trihydroxyl group and outstanding anti-interference ability for its large Stokes shift of 101 nm (lambda(abs) = 435 nm, lambda(em) = 536 nm), thus making the method highly suitable for bioimaging. The probe CF536 was successfully used for fluorescent imaging of copper and hydrogen sulfide in living cells. (C) 2017 Elsevier B.V. All rights reserved.