Ureteric bud cells secrete multiple factors, including bFGF, which rescue renal progenitors from apoptosis
Ureteric bud cells secrete multiple factors, including bFGF, which rescue renal progenitors from apoptosis
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DOI：
10.1152/ajprenal.1997.273.5.f757
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发表时间：
1997-11-01
影响因子：
4.2
通讯作者：
Herzlinger, D
中科院分区：
文献类型：
--
作者：
Barasch, J;Qiao, JZ;Herzlinger, D
肾脏的发育需要输尿管芽和后肾间充质之间的相互作用。间充质的存活和间充质细胞的肾单位的发育取决于来自侵入输尿管芽的信号，而输尿管芽的生长取决于来自间充质的信号。这种相互依赖性使得鉴定由输尿管芽表达的调节间充质生长的分子变得困难。为了确定输尿管芽如何向间充质发出信号，我们先前分离了输尿管芽细胞系（UB细胞）。这些细胞分泌可溶性因子，其拯救间充质免于凋亡。我们现在报告，四个肝素结合因子介导这种生长活性。其中之一是碱性成纤维细胞生长因子（bFGF），它是由输尿管芽在穿透间质时合成的。碱性成纤维细胞生长因子拯救了间充质中发现的三种类型的祖细胞：肾小管上皮细胞的前体、毛细血管的前体和调节输尿管芽生长的细胞。这些数据表明，输尿管芽调节上皮细胞和血管前体细胞的数量，通过分泌bFGF和其他可溶性因子产生肾单位。
Kidney development requires reciprocal interactions between the ureteric bud and the metanephrogenic mesenchyme. Whereas survival of mesenchyme and development of nephrons from mesenchymal cells depends on signals from the invading ureteric bud, growth of the ureteric bud depends on signals from the mesenchyme. This codependency makes it difficult to identify molecules expressed by the ureteric bud that regulate mesenchymal growth. To determine how the ureteric bud signals the mesenchyme, we previously isolated ureteric bud cell lines (UB cells). These cells secrete soluble factors which rescue the mesenchyme from apoptosis. We now report that four heparin binding factors mediate this growth activity. One of these is basic fibroblast growth factor (bFGF), which is synthesized by the ureteric bud when penetrating the mesenchyme. bFGF rescues three types of progenitors found in the mesenchyme: precursors of tubular epithelia, precursors of capillaries, and cells that regulate growth of the ureteric bud. These data suggest that the ureteric bud regulates the number of epithelia and vascular precursors that generate nephrons by secreting bFGF and other soluble factors.