Disruption of the mycobacterial cell entry gene of Mycobacterium bovis BCG results in a mutant that exhibits a reduced invasiveness for epithelial cells
Disruption of the mycobacterial cell entry gene of Mycobacterium bovis BCG results in a mutant that exhibits a reduced invasiveness for epithelial cells
复制标题
DOI：
10.1016/s0378-1097(99)00301-8
复制
发表时间：
1999-08-15
影响因子：
2.1
通讯作者：
Klein, MH
中科院分区：
文献类型：
--
作者：
Flesselles, B;Anand, NN;Klein, MH
属于结核分支杆菌复合体的分支杆菌具有入侵非吞噬细胞并在非吞噬细胞中复制的能力，这一事件需要存在能够触发细胞反应和随后微生物内化的细菌表面成分。在这项研究中，我们报告了牛分枝杆菌卡介苗（BCG）的分枝杆菌细胞进入基因（mce）的测序和产生与失活的mce基因的突变BCG菌株的特性，通过同源重组与双交换。该突变菌株不表达分枝杆菌细胞进入蛋白（Mce），并且与野生型BCG相比，表现出侵入非吞噬上皮细胞系HeLa的能力降低。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in nonphagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report the sequencing of the mycobacterial cell entry gene (mce) of Mycobacterium bovis bacillus Calmette-Guerin (BCG) and the generation and characterization of a mutant BCG strain with an inactivated mce gene, by homologous recombination with double cross-over. This mutant strain does not express the mycobacterial cell entry protein (Mce) and exhibits a reduced ability to invade the non-phagocytic epithelial cell line HeLa as compared to wild-type BCG. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.