LINEAR MITOCHONDRIAL DNAS OF YEASTS - FREQUENCY OF OCCURRENCE AND GENERAL FEATURES
LINEAR MITOCHONDRIAL DNAS OF YEASTS - FREQUENCY OF OCCURRENCE AND GENERAL FEATURES
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DOI：
10.1128/mcb.13.4.2309
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发表时间：
1993-04-01
影响因子：
5.3
通讯作者：
VIOLA, AM
中科院分区：
文献类型：
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作者：
FUKUHARA, H;SOR, F;VIOLA, AM
在大多数酵母物种中，线粒体DNA（mtDNA）已被报道为环状分子。然而，两个案件的线性mtDNA与特定的终端先前已被描述。我们研究了线性形式的线粒体DNA的发生频率之间的酵母脉冲场凝胶电泳。在我们研究的毕赤酵母属和拟Williopsis属的58个物种中，线性mtDNA的频率出乎意料地高。13个物种含有线性mtDNA，证实了限制性内切酶图谱，末端标记，和电子显微镜。对毕赤酵母、Williopsis mrakii和P.jadinii的线粒体DNA进行了详细研究。在每种情况下，左和右末端片段共享同源序列。在末端重复序列之间，线粒体基因的顺序在所有线性mtDNA中是相同的，尽管基因组大小有很大的变化。这种基因顺序的恒定性与酵母圆形线粒体基因组中基因排列的巨大变化形成对比。在几个基因上确定的编码序列与环状mtDNA的编码序列高度同源，这表明这两种形式的mtDNA不是遥远的起源。
In most yeast species, the mitochondrial DNA (mtDNA) has been reported to be a circular molecule. However, two cases of linear mtDNA with specific termini have previously been described. We examined the frequency of occurrence of linear forms of mtDNA among yeasts by pulsed-field gel electrophoresis. Among the 58 species from the genera Pichia and Williopsis that we examined, linear mtDNA was found with unexpectedly high frequency. Thirteen species contained a linear mtDNA, as confirmed by restriction mapping, end labeling, and electron microscopy. The mtDNAs from Pichia pijperi, Williopsis mrakii, and P. jadinii were studied in detail. In each case, the left and right terminal fragments shared homologous sequences. Between the terminal repeats, the order of mitochondrial genes was the same in all of the linear mtDNAs examined, despite a large variation of the genome size. This constancy of gene order is in contrast with the great variation of gene arrangement in circular mitochondrial genomes of yeasts. The coding sequences determined on several genes were highly homologous to those of the circular mtDNAs, suggesting that these two forms of mtDNA are not of distant origins.