Cloning and characterization of the socA locus which restores development to Myxococcus xanthus C-signaling mutants
Cloning and characterization of the socA locus which restores development to Myxococcus xanthus C-signaling mutants
复制标题
恢复黄色粘球菌 C 信号突变体发育的 socA 位点的克隆和表征
DOI：
10.1128/jb.176.8.2200-2209.1994
复制
发表时间：
1994
影响因子：
3.2
通讯作者：
L. Shimkets
中科院分区：
文献类型：
--
作者：
Keesoo Lee;L. Shimkets
csgA基因在粘细菌黄色粘球菌子实体形成期间产生细胞间信号。分离出孢子形成假回复突变体，使我们能够了解CsgA被细胞感知并用于调节发育基因表达的机制。两个菌株，LS 559和LS 560，具有紧密连锁的转座子插入，分别为csp-559（以前的csp-559）和csp-560（以前的csp-560），恢复了csgA突变失去的所有发育行为，包括涟漪，形成子实体和孢子形成的能力。socA基因座的序列分析显示，有三个推定的蛋白质编码区，命名为socA 1，socA 2和socA 3。推测的socA 1的氨基酸序列具有短链醇脱氢酶家族的特征。推导的socA 2的氨基酸序列与普通变形杆菌中将延胡索酸还原酶锚定到膜上的frd操纵子的frdD基因产物具有48%的同一性。推测的socA 3的氨基酸序列与任何已知的蛋白质没有同源性。基因型互补，北方（RNA）印迹，DNA序列分析，和基因表达模式都表明，这三个基因是多顺反子。由于socA突变有效地绕过了CsgA，为什么CsgA在M.通过研究socA孢子的长期稳定性来检查xanthus。与野生型不同，socA突变体孢子在饥饿琼脂上萌发。孢子薄切片的透射电子显微照片显示，发芽是不是由于一个明显的结构缺陷的socA孢子。这些结果表明，socA粘孢子在不利的环境条件下长期存活的能力受到严重影响。因此，soxA在M. xanthus
The csgA gene produces an intercellular signal during fruiting body formation of the myxobacterium Myxococcus xanthus. Sporulating pseudorevertants were isolated to allow us to understand the mechanism by which CsgA is perceived by cells and used to regulate developmental gene expression. Two strains, LS559 and LS560, which have closely linked transposon insertions, soc-559 (formerly csp-559) and soc-560 (formerly csp-560), respectively, regained all the developmental behaviors lost by the csgA mutation including the ability to ripple, form fruiting bodies, and sporulate. The sequence analysis of the socA locus revealed that there are three putative protein-coding regions, designated socA1, socA2, and socA3. The deduced amino acid sequence of socA1 exhibits characteristics of the short-chain alcohol dehydrogenase family. The deduced amino acid sequence of socA2 shares 48% identity with the frdD gene product of the frd operon in Proteus vulgaris which anchors fumarate reductase to the membrane. The deduced amino acid sequence of socA3 does not show homology to any known proteins. Genotypic complementation, Northern (RNA) blotting, DNA sequence analysis, and the pattern of gene expression all suggest that these three genes are polycistronic. Since the socA mutations effectively bypass CsgA, the question of why csgA is maintained in M. xanthus was examined by studying the long-term stability of socA spores. Unlike the wild type, socA mutant spores germinated on starvation agar. Transmission electron micrographs of spore thin sections revealed that germination is not due to an obvious structural deficiency of the socA spores. These results suggest that the ability of socA myxospores to survive long periods under unfavorable environmental conditions is severely comprised. Therefore, soxA appears to be essential for the development of M. xanthus.
黄色粘球菌的乳清苷-5-单磷酸脱羧酶基因。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kimsey,HH;Kaiser,D
通讯作者：
Kaiser,D