Trinucleotide microsatellites in Norway spruce (Picea abies): their features and the development of molecular markers
Trinucleotide microsatellites in Norway spruce (Picea abies): their features and the development of molecular markers
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挪威云杉（Picea abies）的三核苷酸微卫星：其特征和分子标记的开发
DOI：
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发表时间：
2002
影响因子：
5.4
通讯作者：
M. Morgante
中科院分区：
文献类型：
--
作者：
I. Scotti;F. Magni;G. Paglia;M. Morgante
摘要：三核苷酸微卫星已被证明是人类遗传分析的首选标记，因为它们比二核苷酸更容易进行基因分型。由于它们在基因组中的丰度较低，它们的发育可能会更加耗时。我们从挪威云杉(Picea abies K.)用一种富集法构建基因组文库。本文报道了85个ATC微卫星克隆的特征，从中开发出39个标记。许多克隆显示出比三核苷酸重复序列更高级别的串联重复序列，通常类似于小卫星重复序列。对其中一个基因座的等位基因样本进行测序，发现由于微卫星区域内的碱基替换，大小同质性。在重复序列家族中观察到ATC基序的存在。我们发现微卫星的长度与其多态程度之间存在显著的相关性。ATC三核苷酸标记的变异性水平低于二核苷酸标记，无论是在一组六个个体的所有座位上测试时，还是在四个自然群体中的一个座位子集上测试时都是如此。这种差异很可能归因于三核苷酸的突变率低于二核苷酸的突变。具有不同突变率的标记的可用性使人们能够选择合适的标记集来研究不同时间尺度上的群体过程。
Abstract.Trinucleotide microsatellites have proven to be the markers of choice in human genetic analysis because they are easier to genotype than dinucleotides. Their development can be more time-consuming due to their lower abundance in the genome. We isolated trinucleotide microsatellites in Norway spruce (Picea abies K.) using an enrichment procedure for the genomic-library construction. Here we report on the characterisation of 85 ATC microsatellite-containing clones, from which 39 markers were developed. Many of the clones showed the occurrence of tandem repeats of higher order than the trinucleotide ones, often resembling minisatellite repeats. The sequencing of a sample of the alleles at one of the loci revealed size homoplasy due to base substitutions within the microsatellite region. The presence of ATC motifs within repetitive sequence families was observed. We found a significant relationship between the level of polymorphism and the length of the microsatellite. The levels of variability for ATC trinucleotide markers were lower than those for dinucleotides, both when tested on all loci in a set of six individuals and on a subset of loci in four natural populations. This difference is most likely attributable to lower mutation rates for trinucleotide than for dinucleotide loci. The availability of markers with different mutation rates allows one to select the proper marker set to investigate population processes on different time scales.
DOI：
10.1073/pnas.94.3.1041
发表时间：
1997-02-04
影响因子：
11.1
作者：
Chakraborty, R;Kimmel, M;Deka, R
通讯作者：
Deka, R
影响因子：
3.5
作者：
GASTIER, JM;PULIDO, JC;DUYK, GM
通讯作者：
DUYK, GM
三核苷酸重复序列聚集在酿酒酵母的调控基因中。
影响因子：
3.3
作者：
Young,ET;Sloan,JS;VanRiper,K
通讯作者：
VanRiper,K