THE EFFECT OF ANDROGEN ON NITRIC-OXIDE SYNTHASE IN THE MALE REPRODUCTIVE-TRACT OF THE RAT
THE EFFECT OF ANDROGEN ON NITRIC-OXIDE SYNTHASE IN THE MALE REPRODUCTIVE-TRACT OF THE RAT
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DOI：
10.1016/s0015-0282(16)57555-4
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发表时间：
1995-05-01
影响因子：
6.7
通讯作者：
CHANG, TSK
中科院分区：
文献类型：
--
作者：
CHAMNESS, SL;RICKER, DD;CHANG, TSK
目的：探讨男性生殖道一氧化氮合酶活性是否受雄激素调控。设计：测量三组成熟大鼠生殖器官一氧化氮合酶活性：未手术对照，1周阉割，手术时给予T胶囊1周阉割。利用一氧化氮合酶特异性抑制剂n -硝基- l -精氨酸甲酯（L-NAME）证实了一氧化氮合酶活性的存在。结果：去势后，附睾头、体、尾一氧化氮合酶活性分别显著降低88%、73%、54%。去势后，阴茎一氧化氮合酶活性下降45%，一氧化氮合酶蛋白下降57%。在精囊和前列腺外侧，一氧化氮合酶活性在阉割后从对照组的不可检测水平显著增加。去势后凝腺和前列腺腹背侧一氧化氮合酶活性无明显变化。T替代抑制了去势后各器官一氧化氮合酶活性的变化。此外，在所有三个治疗组的每个器官中测量的活性均被L-NAME抑制了约90%。结论：雄激素对男性生殖道一氧化氮合酶活性的影响存在差异。据我们所知，这是第一次在任何组织中显示一氧化氮合酶活性受到雄激素的影响。
Objective: To determine if nitric oxide synthase activity within the male reproductive tract is regulated by androgen.Design: Nitric oxide synthase activity was measured in the reproductive organs of three groups of mature rats: unoperated controls, 1-week castrates, and 1-week castrates given T capsules at the time of surgery. The presence of nitric oxide synthase activity was confirmed by using the nitric oxide synthase-specific inhibitor N-nitro-L-arginine methyl ester (L-NAME).Results: After castration, nitric oxide synthase activity was significantly reduced by 88%, 73%, and 54% in the caput, corpus, and cauda epididymidis, respectively. In the penis, nitric oxide synthase activity decreased 45% and nitric oxide synthase protein decreased 57% after castration. In the seminal vesicle and lateral prostate, nitric oxide synthase activity increased significantly after castration from nondetectable levels in controls. Nitric oxide synthase activity in the coagulating gland and ventral and dorsal prostate did not change after castration. The changes in nitric oxide synthase activity in all organs after castration were prevented by T replacement. Additionally, the activity measured in every organ in all three treatment groups was >90% inhibited by L-NAME.Conclusion: These data demonstrate that androgen differentially affects nitric oxide synthase activity in the male reproductive tract. To the best of our knowledge this is the first time that nitric oxide synthase activity has been shown to be influenced by androgen in any tissue.