A GENETIC-LOCUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI NECESSARY FOR THE PRODUCTION OF ATTACHING AND EFFACING LESIONS ON TISSUE-CULTURE CELLS
A GENETIC-LOCUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI NECESSARY FOR THE PRODUCTION OF ATTACHING AND EFFACING LESIONS ON TISSUE-CULTURE CELLS
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DOI：
10.1073/pnas.87.20.7839
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发表时间：
1990-10-01
影响因子：
11.1
通讯作者：
KAPER, JB
中科院分区：
文献类型：
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作者：
JERSE, AE;YU, J;KAPER, JB
肠源性大肠埃希菌(EPEC)形成附着和消除肠道病变的能力是EPEC致病的主要特征。利用TnPhoA突变，我们已经确定了这一活动所必需的染色体基因(eae，用于大肠杆菌的附着和消除)。来自该基因的DNA探针与EPEC血清群中的大肠杆菌100%杂交，这些血清群显示出对组织培养细胞的附着和消除活性，以及其他产生附着和消除肠道病变的致病大肠杆菌，如RDEC-1(断奶仔兔的EPEC)和肠出血性大肠杆菌。从EAE的核苷酸序列预测的氨基酸序列与假结核耶尔森菌入侵的氨基酸序列具有显著的同源性。
The ability of enteropathogenic Escherichia coli (EPEC) to form attaching and effacing intestinal lesions is a major characteristic of EPEC pathogenesis. Using TnphoA mutagenesis we have identified a chromosomal gene (eae, for E. coli attaching and effacing) that is necessary for this activity. A DNA probe derived from this gene hybridizes to 100% of E. coli of EPEC serogroups that demonstrate attaching and effacing activity on tissue culture cells as well as other pathogenic E. coli that produce attaching and effacing intestinal lesions, such as RDEC-1 (an EPEC of weanling rabbits) and enterohemorrhagic E. coli. The predicted amino acid sequence derived from the nucleotide sequence of eae shows significant homology to that of the invasion of Yersinia pseudotuberculosis.