A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
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DOI：
10.1007/978-1-4939-7459-7_18
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Tse-Dinh, Yuk-Ching
中科院分区：
文献类型：
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作者：
Annamalai, Thirunavukkarasu;Cheng, Bokun;Tse-Dinh, Yuk-Ching
细菌拓扑异构酶I是一个潜在的目标，为确定新的拓扑异构酶的毒药抑制剂，可以提供线索，一类新的抗菌化合物。在这里，我们详细描述了一种基于荧光的切割试验，成功地用于高温超导发现细菌拓扑异构酶I毒药。
Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase I poisons.