Induction of intestinal tumors and lymphomas in C57BL/6N mice by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
Induction of intestinal tumors and lymphomas in C57BL/6N mice by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
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DOI：
10.1111/j.1349-7006.2002.tb01281.x
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发表时间：
2002-05-01
期刊：
影响因子：
--
通讯作者：
Nakagama, H
中科院分区：
文献类型：
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作者：
Ochiai, M;Imai, H;Nakagama, H
邻菲咯烷胺(2-Amino-1-methyl-6-phenylimidazo[4，5-b]pyridine，PhIP)是熟肉和鱼中含量最丰富的杂环胺。虽然PUP已被证明可以在大鼠身上诱发各种类型的肿瘤，但使用CDF1品系的小鼠中淋巴瘤占主导地位。为了研究PhIP对不同遗传背景小鼠其他器官的致癌作用，将PhIP应用于C57BL/6N小鼠。C57BL/6N小鼠在高脂饲料中灌胃300ppm MAP 40周后，在第95周和第70周时发生小肠腺瘤和腺癌，雄性发生率为52%，雌性为68%。B细胞起源的淋巴瘤在两性中的发病率也与CDF1株一样高，男性发病率为48%，女性发病率为32%。尽管服用PhIP的雌性小鼠的发病率与对照组小鼠没有区别，但服用PhIP的小鼠发生淋巴瘤的时间明显更早。本研究表明，肠道是PhIP诱发小鼠致癌的另一个潜在靶点，PUP的致癌活性可能受到动物遗传背景的影响。
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant heterocyclic amine contained in cooked meat and fish. Although PUP has been demonstrated to induce various types of tumors in rats, lymphomas predominated in mice using the CDF1 strain. To investigate the carcinogenic activity of PhIP on other organs in mice with a different genetic background, PhIP was administered to C57BL/6N mice. After a 40-week administration of 300 ppm of MAP in a high-fat diet followed by continuous feeding with a high fat diet, C57BL/6N mice developed adenomas and adenocarcinomas in the small intestine, the incidences being 52% in males and 68% in females at weeks 95 and 70, respectively. Lymphomas of B-cell origin also developed in both sexes as frequently as in the CDF1 strain, incidences being 48% in males and 32% in females. Although the incidence in PhIP-treated female mice did not differ from that in the control mice, lymphomas developed significantly earlier in the PhIP-treated mice. The present study demonstrated that the intestinal tract is another potential target of PhIP-induced carcinogenesis in mice, and that the carcinogenic activity of PUP could be affected by the genetic background of the animals.