DELETION MUTANTS OF HERPESVIRUS SAIMIRI DEFINE AN OPEN READING FRAME NECESSARY FOR TRANSFORMATION
DELETION MUTANTS OF HERPESVIRUS SAIMIRI DEFINE AN OPEN READING FRAME NECESSARY FOR TRANSFORMATION
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DOI：
10.1128/jvi.63.8.3307-3314.1989
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发表时间：
1989-08-01
影响因子：
5.4
通讯作者：
DESROSIERS, RC
中科院分区：
文献类型：
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作者：
MURTHY, SCS;TRIMBLE, JJ;DESROSIERS, RC
在疱疹病毒saimiri独特的（L-）DNA的左端的5,549个碱基对序列的分析揭示了两个开放的阅读框架和五个小的核U RNA（疱疹病毒saimiri U RNA）的基因。复制能力的缺失突变体的构建，以评估这些遗传特征的重要性，这种致癌疱疹病毒的转化。虽然不需要复制，开放的阅读框架之一被发现是所需的绒猴T淋巴细胞永生化成连续生长的细胞系。通过该阅读框架预测的蛋白质（STP; saimiri转化相关蛋白）具有足以在其羧基末端附近形成跨膜结构域的26个氨基酸的高度疏水性延伸;该结构域之前紧接着适合形成金属结合结构域的序列（His X2 His X6 Cys X2 Cys，其中X是其他氨基酸）。可以编码STP的两个poly（A）+RNA之一是双顺反子的，而另一个具有长的5“非翻译区（. 1.5酶）。虽然STP和EB病毒的淋巴细胞膜蛋白（LMP）之间可以得出一些相似性，但STP与LMP在序列上不相关。
Analysis of a 5,549-base-pair sequence at the left end of herpesvirus saimiri unique (L-) DNA revealed two open reading frames and genes for five small nuclear U RNAs (herpesvirus saimiri U RNAs). Replication-competent deletion mutants were constructed in order to assess the importance of these genetic features for transformation by this oncogenic herpesvirus. Although not required for replication, one of the open reading frames was found to be required for immortalization of marmoset T lymphocytes into continuously growing cell lines. The protein predicted by this reading frame (STP; saimiri transformation-associated protein) has a highly hydrophobic stretch of 26 amino acids sufficient for a membrane-spanning domain near its carboxy terminus; this domain is immediately preceded by a sequence appropriate for formation of a metal-binding domain (His X2 His X6 Cys X2 Cys, where Xs are other amino acids). One of two poly(A)+ RNAs that could encode STP is bicistronic, while the other has a long 5'' untranslated region (.apprx. 1.5 kilobases). Although some analogies can be drawn between STP and LMP (lymphocyte membrane protein) of Epstein-Barr virus, STP is not related in sequence to LMP.