Role of viability of probiotic strains in their persistence in the gut and in mucosal immune stimulation
Role of viability of probiotic strains in their persistence in the gut and in mucosal immune stimulation
复制标题
DOI：
10.1111/j.1365-2672.2004.02353.x
复制
发表时间：
2004-01-01
影响因子：
4
通讯作者：
Perdigón, G
中科院分区：
文献类型：
--
作者：
Galdeano, CM;Perdigón, G
目的：确定益生菌如何与肠道上皮细胞和免疫细胞接触以及诱导良好粘膜免疫刺激的条件。方法和结果：通过透射电子显微镜（TEM）研究干酪乳杆菌，以确定其与肠道的相互作用。我们比较了活的和非活的乳酸菌对肠粘膜免疫系统（IMIS）的影响及其在小鼠肠道中的持久性。TEM显示整个Lact。干酪素粘附在绒毛上，在肠上皮细胞的细胞质中发现细菌抗原。活菌刺激的综管系统在更大程度上比非活菌与乳酸菌除外。德氏保加利亚人。结论：抗原颗粒能进入肠上皮细胞并与免疫细胞接触，而全菌不能。细菌活力是一个更好的刺激IMSIS.Significance和影响的研究的条件：我们证明，只有抗原颗粒与免疫细胞相互作用，它们从肠道的快速清除与颗粒抗原所描述的一致。定期食用益生菌不应对宿主产生不利影响。
Aims: To determine how probiotic bacteria contact with intestinal epithelial and immune cells and the conditions to induce a good mucosal immune stimulation.Methods and Results: Lactobacillus casei was studied by transmission electron microscopy (TEM) to determine its interaction with the gut. We compared the influence of viable and nonviable lactic acid bacteria on the intestinal mucosal immune system (IMIS) and their persistence in the gut of mice. TEM showed whole Lact. casei adhered to the villi; the bacterial antigen was found in the cytoplasm of the enterocytes. Viable bacteria stimulated the IMIS to a greater extent than nonviable bacteria with the exception of Lact. delbrueckii subsp. bulgaricus. For all the strains assayed at 72 h no antigenic particles were found in the intestine.Conclusion: Antigenic particles but not the whole bacteria can enter to epithelial cells and contact with the immune cells. Bacterial viability is a condition for a better stimulation of the IMIS.Significance and Impact of the Study: We demonstrated that only antigenic particle interact with the immune cells and their fast clearance from the gut agrees with those described for the particulate antigens. The regular consumption of probiotics should not adversely affect the host.