CHANGES IN THE ARGINASE AND ALKALINE PHOSPHATASE CONTENTS OF THE MAMMARY GLAND AND LIVER OF THE RAT DURING PREGNANCY, LACTATION AND MAMMARY INVOLUTION
CHANGES IN THE ARGINASE AND ALKALINE PHOSPHATASE CONTENTS OF THE MAMMARY GLAND AND LIVER OF THE RAT DURING PREGNANCY, LACTATION AND MAMMARY INVOLUTION
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DOI：
10.1042/bj0410261
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发表时间：
1947-01-01
影响因子：
4.1
通讯作者：
GREENBAUM, AL
中科院分区：
文献类型：
--
作者：
FOLLEY, SJ;GREENBAUM, AL
研究了大鼠在妊娠期、哺乳期和乳房退化期乳腺和肝脏碱性磷酸酶以及乳腺和肾脏碱性磷酸酶的变化。通过测定乳腺匀浆中的乳糖来估计乳腺组织中的残留乳汁，并将其用于校正乳腺酶测定值。变成真正的湿润组织肝纤维化酶含量（/g.湿组织）与未交配的对照组相比，除了在妊娠第15天观察到显著较低的值外，没有区别。在整个哺乳期，肝脏脂肪酶显着增加高于这些值。乳腺过氧化物酶在妊娠期和泌乳早期缓慢增加，但在泌乳的第5天和第10天之间急剧增加。它在哺乳期第20天达到最大值，断奶后急剧下降到妊娠早期的水平。乳腺中的碱性磷酸酶含量在怀孕期间迅速增加，在分娩时达到最大值，并在整个哺乳期保持在同一水平;断奶后下降到哺乳期和早孕期之间的中间水平。肾组织的碱性磷酸酶含量在整个妊娠期、哺乳期和乳房退化期保持不变。结果表明，乳腺脱氢酶与乳腺对氨基酸的脱氨基作用有关，大鼠泌乳后期脱氢酶含量的增加是由于合成乳成分所需的脱氨基残留物增加。这一结果与乳腺碱性磷酸酶可能与生长期核蛋白的合成有关，也可能与泌乳期酪蛋白的合成和血糖分子的捕获有关的观点是一致的。在哺乳期大鼠，肾脏组织包含在平均。2-9是乳腺组织碱性磷酸酶的两倍在乳腺生长阶段，即在怀孕早期，肾脏/乳腺的比例约为。34.
Changes in the mammary gland and liver arginase, and the mammary gland and kidney alkaline phosphatase, were studied in pregnancy, lactation, and mammary involution in the rat. Retained milk in mammary gland tissue was estimated by determining lactose in the gland homogenates and this used to correct the mammary gland enzyme detns. to a true moist tissue basis. The liver arginase content (/g. moist tissue) during pregnancy and after weaning was indistinguishable from that of unmated controls except at the 15th day of pregnancy when significantly lower values were observed. Over the whole lactation period, the liver arginase was significantly increased above those values. The mammary gland arginase increased slowly during pregnancy and early lactation but underwent a dramatic increase between the 5th and 10th days of lactation. It reached a maximum at the 20th day of lactation and after weaning fell precipitously to the level of early pregnancy. The alkaline phosphatase content in the mammary gland increased rapidly during pregnancy, reaching a maximum at parturition, and remaining at the same level throughout lactation; it decreased after weaning to a level intermediate between those of lactation and early pregnancy. The alkaline phosphatase content of kidney tissue remained constant throughout pregnancy, lactation, and mammary involution. The results were believed to indicate that the mammary gland arginase was concerned with the deamination of amino-acids by the mammary gland, and that the increased arginase content during late lactation in the rat was due to an increased requirement of deaminized residues for the synthesis of milk constituents. The results were consistent with the view that the alkaline phosphatase of the mammary gland might be concerned with the synthesis of nucleo-proteins during the growth phase and possibly with the synthesis of casein and the capture of blood sugar molecules during lactation. During lactation in the rat, kidney tissue contained on the avg. 2-9 times as much alkaline phosphatase as mammary gland tissue. During the mammary growth phase, i.e. in early pregnancy, the kidney/mammary gland ratio was approx. 34.