Morphological, structural, and functional characterization of the haemocytes of the scallop, Argopecten irradians
Morphological, structural, and functional characterization of the haemocytes of the scallop, Argopecten irradians
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DOI：
10.1016/j.aquaculture.2005.05.020
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发表时间：
2006-01
期刊：
影响因子：
4.5
通讯作者：
Weizhu Zhang;Xinzhong Wu;Ming Wang
中科院分区：
文献类型：
--
作者：
Weizhu Zhang;Xinzhong Wu;Ming Wang
海湾扇贝是我国扇贝科最重要的经济品种之一。软体动物的内部防御系统由循环血细胞组成。为了表征扇贝A. irradians 的血细胞，进行了光和电子显微镜研究。识别出四种类型的血细胞：I 型小透明细胞（2.38±0.08 μm，30-35%）、II 型大透明细胞（4.41±0.33 μm，15-20%）、III 型小粒细胞（4.15±0.26 μm，20-25%）和 IV 型大粒细胞（8.26±0.52 μm，15-20%）。 25–30%）。粒细胞类型的 N/C 比值比透明细胞小。血淋巴平均血细胞浓度约为(3.75±0.65)×107cells ml−1。血细胞中，44.7%为颗粒状，55.3%为无颗粒状。这些给出了辐照弧菌血细胞相对系统的分类方案。确定了三种类型的颗粒： I 型，具有高电子密度； II型，电子密度低；根据 TEM 研究，III 型具有中等电子密度。本研究中未使用Percoll的DDGC分离不同类型的血细胞。粒细胞和透明细胞均对大肠杆菌和 RLO 两种细菌菌株表现出吞噬反应。粒细胞的吞噬能力(41-48%)显着高于透明细胞(9.2-11.2%)。
Argopecten irradians is one of the most important commercial species of Pectinidae family in China. The internal defense system of mollusks consists of circulating haemocytes. In order to characterize the haemocytes of the scallop A. irradians, light and electron microscopical studies were carried out. Four types of haemocytes were recognized: type I small hyalinocytes (2.38±0.08 μm, 30–35%), type II large hyalinocytes (4.41±0.33 μm, 15–20%), type III small granulocytes (4.15±0.26 μm, 20–25%), and type IV large granulocytes (8.26±0.52 μm, 25–30%). Granulocyte types showed smaller N/C ratios than hyalinocytes. The mean haemocyte concentration was about (3.75±0.65)×107cells ml−1of haemolymph. Among haemocytes, 44.7% are granular and 55.3% are agranular. These gave a relatively systematic classification scheme for haemocytes of A. irradians. Three types of granules were identified: type I, with high electron-density; type II, with low electron-density; and type III, with a middle level of electron-density, based on TEM studies. Different haemocyte types were not separated with DDGC of Percoll in this study. Both granulocytes and hyalinocytes showed a phagocytic response to the two strains of bacteria, Escherichia coli and RLOs. The phagocytic ability of granulocyte was significantly higher (41–48%) than that of hyalinocyte (9.2–11.2%).