Immunocytochemistry of human brain tissue with a polyclonal antiserum against neuropeptide Y.
Immunocytochemistry of human brain tissue with a polyclonal antiserum against neuropeptide Y.
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使用抗神经肽 Y 的多克隆抗血清对人脑组织进行免疫细胞化学分析。
DOI：
10.1007/bf00318333
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Yasargil,G
中科院分区：
文献类型：
--
作者：
Chan-Palay,V;Yasargil,G
用免疫细胞化学方法比较手术组织样本和死后材料中含有npy的神经元结构。然而，在手术标本中，单个神经元和神经轴突和树突丛的保存质量异常良好。这一结果很可能是由于在手术切除期间和之后快速而仔细地处理组织可以定期获得良好的固定。该组织适合于光显微镜和电子显微镜，并且优越的保存也导致强烈，可靠的抗体反应。如前所述，如果处理得当，死后组织样本可以为免疫细胞化学提供良好的标本。然而，在固定之前的死后延迟破坏了免疫染色结构中的神经元完整性。然而，与年龄匹配的对照组相比，仔细研究神经系统疾病受试者的死后材料可以提供有价值的信息。
NPY-containing neuronal structures in the cereoral cortex of surgical tissue samples were compared to those in postmortem material by immunocytochemical methods. However, the quality of preservation of individual neurons and axonal and dendritic plexuses in the neuropil is unusually fine in the surgical specimens. This result is most likely attributable to the excellent fixation that can be regularly achieved by rapid and careful handling of tissue during and after surgical removal. The tissue is suitable for both light and electron microscopy, and the superior preservation also leads to intense, reliable antibody reactions. Postmortem tissue samples can provide good specimens for immunocytochemistry when properly handled as previously described. However the postmortem delays prior to fixation disrupt neuronal integrity in the immunostained structures. Nevertheless, postmortem material from carefully studied subjects of neurological diseases compared with age matched controls can provide valuable information.