Molecular evidence of allodiploidy in F1 gametophytic blades from a cross between Neopyropia yezoensis and a cryptic species of the Neopyropia yezoensis complex (Bangiales, Rhodophyta) by the use of microsatellite markers
Molecular evidence of allodiploidy in F1 gametophytic blades from a cross between Neopyropia yezoensis and a cryptic species of the Neopyropia yezoensis complex (Bangiales, Rhodophyta) by the use of microsatellite markers
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DOI：
10.1016/j.aqrep.2020.100489
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发表时间：
2020-11
影响因子：
3.7
通讯作者：
K. Niwa;F. Sano;T. Sakamoto
中科院分区：
文献类型：
--
作者：
K. Niwa;F. Sano;T. Sakamoto
我们研究了先前为两种海洋作物Neopyropia yezoensis和Neopyropia tenera开发的6个微卫星（MS）标记是否可用于Neopyropia yezoensis和neopyropiasp杂交产生的配子体叶片的分子分析。2，一种隐种；yezoensiscomplex。6个MS标记中的3个（Pye1、Pye13和pye38）可用于隐种neopyropiasp的基因分型。2. 利用这三个MS标记，我们评估了f1配子体叶片是否由n。yezoensis和隐种neopyropiasp。其中2个在单倍体配子体期是异源二倍体。在两个MS位点（pye13和pye38）上均表现出杂合的基因型。MS标记分析清楚地表明f1叶片是异源二倍体，有力地支持了先前基于单个CAPS标记的研究结果。此外，本研究使用的MS标记可以作为研究异源多倍体和同域物种形成的有用工具。
We investigated whether six microsatellite (MS) markers previously developed for two marine crop species,Neopyropia yezoensisandNeopyropia tenera(formerlyPyropia yezoensisandPyropia tenera, respectively), are useful for molecular analysis of gametophytic blades resulting from a cross betweenNeopyropia yezoensisandNeopyropiasp. 2, a cryptic species of theN. yezoensiscomplex. Three (Pye1,Pye13, andPye38) of the six MS markers could be used to genotype the cryptic speciesNeopyropiasp. 2. Using these three MS markers, we evaluated whether the F1gametophytic blades resulting from the cross betweenN. yezoensisand the cryptic speciesNeopyropiasp. 2 are allodiploid in the haploid gametophytic phase. The F1blades that developed from both conchospore and archeospore exhibited heterozygous genotypes at two MS loci (Pye13andPye38). The MS marker analyses clearly showed that the F1blades were allodiploid, strongly supporting the findings of a previous study that was based on a single CAPS marker. In addition, the MS markers employed in this study could serve as useful tools for studies on allopolyploidy in and sympatric speciation of the marine algaNeopyropia.