An integrated physical and genetic map of a 35 Mb region on chromosome Xp22.3-Xp21.3.
An integrated physical and genetic map of a 35 Mb region on chromosome Xp22.3-Xp21.3.
复制标题
染色体 Xp22.3-Xp21.3 上 35 Mb 区域的综合物理和遗传图谱。
DOI：
10.1093/hmg/4.10.1821
复制
发表时间：
1995
影响因子：
3.5
通讯作者：
Ballabio,A
中科院分区：
文献类型：
--
作者：
Ferrero,GB;Franco,B;Roth,EJ;Firulli,BA;Borsani,G;Delmas-Mata,J;Weissenbach,J;Halley,G;Schlessinger,D;Chinault,AC;Zoghbi,HY;Nelson,DL;Ballabio,A
我们已经构建了跨越人类染色体Xp22的35Mb区域的详细物理图谱。3-Xp21。3.图谱的主干由覆盖整个区域的585个重叠的酵母人工染色体(YAC)克隆的单一定向连续延伸表示。该图谱由615个图谱对象组成，包括324个YAC、185个序列标记点、28个基因、85个染色体断裂点和37个高度多态标记。使用由染色体断裂点和YAC克隆的重叠区域定义的183个条带来指导和确认物理作图。多态标记在物理地图中的定位使整个区域的物理和遗传数据得以整合。这些数据建立了染色体Xp22。3-Xp21。3是人类基因组中最具特征的大区域之一。该图谱将极大地促进更精细的比例尺测绘和测序，以及从人类基因组的这一部分识别疾病基因。
We have constructed a detailed physical map of the 35 Mb region spanning human chromosome Xp22. 3-Xp21. 3. The backbone of the map is represented by a single oriented contiguous stretch of 585 overlapping yeast artificial chromosome (YAC) clones covering the entire region. The map is formatted with 615 map objects that include 324 YACs, 185 sequence tagged sites, 28 genes, 85 chromosomal breakpoints and 37 highly polymorphic markers. Physical mapping was both guided and confirmed using 183 bins defined by chromosomal breakpoints and by overlapping regions of YAC clones. The localization of polymorphic markers in the physical map permits the integration of physical and genetic data across the region. These data establish chromosome Xp22. 3-Xp21. 3 as one of the best characterized large regions in the human genome. The map should greatly facilitate finer scale mapping and sequencing as well as the identification of disease genes from this portion of the human genome.