Silencing of long noncoding RNA SBF2-AS1 inhibits proliferation, migration and invasion and contributes to apoptosis in osteosarcoma cells by upregulating microRNA-30a to suppress FOXA1 expression (Retracted article. See MAR, 2023)
Silencing of long noncoding RNA SBF2-AS1 inhibits proliferation, migration and invasion and contributes to apoptosis in osteosarcoma cells by upregulating microRNA-30a to suppress FOXA1 expression (Retracted article. See MAR, 2023)
复制标题
DOI：
10.1080/15384101.2019.1656478
复制
发表时间：
2019-08-22
期刊：
影响因子：
4.3
通讯作者：
Luo, Jun
中科院分区：
文献类型：
--
作者：
Dai, Jiang-Hua;Huang, Wen-Zhou;Luo, Jun
目的：长链非编码RNA（lncRNA）SBF 2-AS 1与某些肿瘤相关。然而，SBF 2-AS 1在骨肉瘤（OS）中的作用仍需进一步阐明。本研究旨在检测lncRNA SBF 2-AS 1在OS中的表达，以及microRNA-30 a（miR-30 a）和FOXA 1的参与。研究方法：取骨肉瘤组织及相应癌旁正常组织，检测SBF 2-AS 1的表达及其与临床表型的关系。选择SBF 2-AS 1表达最显著的OS细胞用于后续实验。并通过一系列实验检测OS细胞的增殖、侵袭、迁移、集落形成、细胞周期分布及凋亡情况。进一步验证了SBF 2-AS 1与miR-30 a以及miR-30 a与FOXA 1的结合位点。结果如下：SBF 2-AS 1在OS组织和细胞中过表达，沉默SBF 2-AS 1和miR-30 a过表达可抑制OS细胞的增殖、迁移和侵袭，促进其凋亡。此外，lncRNA SBF 2-AS 1通过充当ceRNA来调节miR-30 a，从而促进FOXA 1表达。结论：沉默SBF 2-AS 1可抑制OS细胞的增殖、迁移和侵袭，并通过与miR-30 a结合，抑制FOXA 1的表达，促进OS细胞凋亡。
Objectives: Long noncoding RNA (lncRNA) SBF2-AS1 was found to be related to some tumors. Nevertheless, the role of SBF2-AS1 in osteosarcoma (OS) is still needed to be elaborated. This study is conducted to examine the expression of lncRNA SBF2-AS1 in OS with the involvement of microRNA-30a (miR-30a) and FOXA1. Methods: OS tissues and its corresponding adjacent normal tissues were obtained for the detection of SBF2-AS1 expression and its relations with clinical phenotypes. OS cells with most significant expression of SBF2-AS1 were selected for subsequent experiments. Moreover, a series of experiments were performed to detect proliferation, invasion, migration, colony formation, cell cycle distribution and apoptosis of OS cells. Furthermore, the binding site between SBF2-AS1 and miR-30a as well as between miR-30a and FOXA1 was verified. Results: SBF2-AS1 was overexpressed in tissues and cells of OS. Additionally, silencing of SBF2-AS1 and miR-30a overexpression inhibited the proliferation, migration and invasion of OS cells and promoted their apoptosis. Moreover, lncRNA SBF2-AS1 regulated miR-30a by serving as a ceRNA, thus promoting FOXA1 expression. Furthermore, interfered SBF2-AS1 or upregulated miR-30a restrained the growth of OS. Conclusion: Our study confirms that silencing of SBF2-AS1 represses proliferation, migration and invasion of OS cells and promotes their apoptosis by binding to miR-30a and inhibiting FOXA1 expression.