Origins of bidirectional promoters: Computational analyses of intergenic distance in the human genome
Origins of bidirectional promoters: Computational analyses of intergenic distance in the human genome
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DOI：
10.1093/molbev/msh040
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发表时间：
2004-03-01
影响因子：
10.7
通讯作者：
Jones, PA
中科院分区：
文献类型：
--
作者：
Takai, D;Jones, PA
我们分析了基因间距离，并使用人类染色体20，21和22的完整序列和映射信息搜索双向基因的存在，其中包含2,122个已知和预测的基因。不同转录本基因间的基因间距呈双相分布，强峰为25 kb，弱峰为0.3kb，表明不同转录本基因可能有共同的启动子。在基因的转录末端没有观察到弱峰。73%（55/75对基因，从总共150个基因）的这些不同的转录本位于1 kb内的另一个是CpG岛。不同的转录本基因的表达在不同的人体组织中并不一致，这表明它们是独立调节的。分析基因间序列中散布重复序列的出现频率表明，这些重复序列被强烈排除在转录起始区域之外。这种排除可能是这些不同的转录本存在的原因。
We have analyzed intergenic distances and searched for the presence of bidirectional genes using the complete sequences and mapping information of human chromosomes 20, 21, and 22, which contain 2,122,known and predicted genes. Intergenic distances between genes with divergent transcripts were distributed in a biphasic manner with a strong peak of 25 kb and a weak peak of 0.3 kb between the divergent transcripts, suggesting that the genes might share a common promoter. The weak peak was not observed at the transcriptional ends of genes. Seventy-three percent (55/75 pairs of genes, from a total of 150 genes) of these divergent transcripts located within 1 kb of one another were CpG islands. Expression of the divergent transcript genes was not concordant in various human tissues, suggesting that they were independently regulated. Analyses of the frequency of occurrence of interspersed repeats in the intergenic sequences suggested that these repeats are strongly excluded from the regions of transcriptional starts. This exclusion might be responsible for the existence of these divergent transcripts.