Folding dynamics of single GCN4 peptides by fluorescence resonant energy transfer confocal microscopy
Folding dynamics of single GCN4 peptides by fluorescence resonant energy transfer confocal microscopy
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DOI：
10.1016/s0301-0104(99)00127-5
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发表时间：
1999-08-01
期刊：
影响因子：
2.3
通讯作者：
Hochstrasser, RM
中科院分区：
文献类型：
--
作者：
Jia, YW;Talaga, DS;Hochstrasser, RM
我们已经制备了一种用于单分子荧光能量转移实验（GCN 4-Pf）的GCN 4-P1的双色交联变体。单GCN 4-Pf分子的折叠和去折叠的波动测量在一个双通道的共聚焦显微镜与供体和受体的荧光轨迹同时测量。由此确定了能量转移效率，并计算了其概率分布作为加入变性剂[尿素]的函数。分布表明，单分子GCN 4-Pf处于动态折叠平衡，平衡位置被尿素浓度改变。(C)1999 Elsevier Science B. V.保留所有权利。
We have prepared a bichromophoric crosslinked variant of GCN4-P1 for single-molecule fluorescence energy transfer experiments (GCN4-Pf). The folding and unfolding fluctuations of single GCN4-Pf molecules are measured in a two-channel confocal microscope with which donor and acceptor fluorescence trajectories are measured simultaneously. The energy transfer efficiency is thereby determined and its probability distributions as a function of added denaturant [urea] are calculated The distributions indicate that single molecule GCN4-Pf is in dynamic folding equilibrium with the position of the equilibrium being altered by the concentration of urea. (C) 1999 Elsevier Science B.V. All rights reserved.