A homogeneous immunoassay for detection of the interaction between two tumor biomarkers of IGF1R-β and SOCS1
A homogeneous immunoassay for detection of the interaction between two tumor biomarkers of IGF1R-β and SOCS1
复制标题
用于检测 IGF1R-β 和 SOCS1 两种肿瘤生物标志物之间相互作用的均相免疫分析
DOI：
10.1002/bab.1989
复制
发表时间：
2020-08-04
影响因子：
2.8
通讯作者：
Hao，Wenbo
中科院分区：
文献类型：
--
作者：
Kuang，Zhenzhan;Deng，Hao;Hao，Wenbo
The current protein interaction method is time consuming and cumbersome or the instrument is expensive. A new method that is convenient, fast, and high throughput needs to be studied urgently. The purpose of this study was to establish a homogeneous immunoassay to detect the interaction between insulin‐like growth factor‐1 receptor‐β (IGF1R‐β) and suppressor of cytokine signaling 1 (SOCS1). The recombinant vectors IGF1R‐β/pENTER and SOCS1/pENTER were constructed and transfected into 293T cells. Based on homogeneous immunoassay technology, we established a suitable method. The signal intensity in the 293T lysate that overexpressed IGF1R‐β and SOCS1, respectively, was compared with the signal intensity in the simultaneous expression of IGF1R‐β and SOCS1. The interaction between IGF1R‐β and SOCS1 was verifiedin vitro. The detection system for the interaction between IGF1R‐β and SOCS1 was established. Compared with other methods, homogeneous immunoassay has the advantages of being rapid and sensitive, having higher sensitivity, and easy to operate. The interaction between IGF1R‐β and SOCS1 was tested to verify the feasibility of this method and prove its practicability and sensitivity. This new method can be used as a high‐throughput platform for protein–protein interaction, with the advantages of trace detection, short detective time, and high detective sensitivity.
