A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cells.
A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cells.
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RAD9 依赖性检查点可阻断 cdc13 酵母细胞的减数分裂。
DOI：
10.1093/genetics/131.1.55
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Byers,B
中科院分区：
文献类型：
--
作者：
Weber,L;Byers,B
先前已经发现CDC 13中的突变导致酿酒酵母的细胞周期停滞在紧接有丝分裂之前的G2阶段。我们在这里表明，cdc13在DNA复制后的一个阶段阻断了减数分裂途径，但在这种情况下，纺锤体尚未形成，染色体也没有发生突触或重组。这种停滞被rad9缓解，因此暗示了当存在染色体病变时延迟有丝分裂进展的相同检查点功能。缓解减数分裂阻滞的rad9后产生的孢子的评估表明，在单独携带cdc13的菌株中不存在重组是由于RAD9介导的阻滞，而不是cdc13病变的其他影响。我们已经测试了这种检查点功能在调节减数分裂进程中重要的可能性，以允许在正在进行的减数分裂期间解决重组中间体，并且没有发现rad9改变可能依赖于这种调节的功能的执行的证据。我们考虑了其他检查点在酵母减数分裂中的可能作用。
Mutations in CDC13 have previously been found to cause cell cycle arrest of Saccharomyces cerevisiae at a stage in G2 immediately preceding the mitotic division. We show here that cdc13 blocks the meiotic pathway at a stage that follows DNA replication, but in this case the spindle has not yet formed nor have the chromosomes undergone synapsis or recombination. This arrest is alleviated by rad9, thus implicating the same checkpoint function that delays mitotic progression when chromosomal lesions are present. An assessment of the spores produced upon alleviation of the meiotic arrest by rad9 reveals that the absence of recombination in strains bearing cdc13 alone is attributable to the RAD9-mediated arrest rather than to other effects of cdc13 lesions. We have tested the possibility that this checkpoint function is important in regulating meiotic progression to permit resolution of recombinational intermediates during ongoing meiosis and have found no evidence that rad9 alters the execution of functions that might depend upon such regulation. We consider the possible role of other checkpoints in yeast meiosis.