COULOMETRIC TOTAL CARBON-DIOXIDE ANALYSIS FOR MARINE STUDIES - AUTOMATION AND CALIBRATION
COULOMETRIC TOTAL CARBON-DIOXIDE ANALYSIS FOR MARINE STUDIES - AUTOMATION AND CALIBRATION
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DOI：
10.1016/0304-4203(87)90033-8
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发表时间：
1987-07-01
期刊：
影响因子：
3
通讯作者：
BRANDSTROM, L
中科院分区：
文献类型：
--
作者：
JOHNSON, KM;SIEBURTH, JM;BRANDSTROM, L
在约翰逊等人的手动样机基础上，研制了一种库仑法测定总二氧化碳（TCO 2）的自动化仪器，用纯CO2校准检测器，整个分析过程由微机控制，装置紧凑，适合野外和船上使用。对波罗的海阿斯克观测站的67个样品（盐度= 6; TCO 2范围为1224.3 - 1276.1 μmol l− 1，平均值为1245.6 μmol l−1）和瑞典湖泊的31个样品（TCO 2范围为19.5 - 252.3 μmol l− 1，平均值为72.9 μmol l−1）进行了分析。这些分析的合并标准偏差为± 1.0 μmol l−1（% CV分别为0.08%和1.3%）。对于超过1000 μmol l− 1 TCO 2的沃茨，< 0.1%的精确度是常规的。在波罗的海的Askö，22个平行样品中TCO 2的库仑法和pH-碱度测定之间的平均绝对差为6.7 μmol kg− 1或平均值的0.5%。群落代谢研究给出的最大净生产率（TCO 2吸收率）和呼吸率（TCO 2生产率）分别为0.9-1.2和0.3-0.5 μmol l−1b−1。这项研究的结果提供了解决方案，观察到的问题与早期的手动原型。
An automated instrument for the coulometric detection of total carbon dioxide (TCO2) was developed from the manual prototype of Johnson et al. Pure CO2was used to calibrate the detector, the whole analytical procedure was controlled by a microcomputer, and the unit was compact enough for field and shipboard use. Some 67 samples from the Askö field station on the Baltic Sea (salinity = 6; TCO2ranging from 1224.3 to 1276.1 μmol l−1with a mean of 1245.6 μmol l−1) and 31 samples from Swedish lakes (TCO2ranging from 19.5 to 252.3 μmol l−1with a mean of 72.9 μmol l−1) were analyzed. The pooled standard deviation of these analyses was ± 1.0 μmol l−1(% CV of 0.08 and 1.3%, respectively). Precisions of < 0.1% were routine for waters exceeding 1000 μmol l−1TCO2. At Askö on the Baltic Sea, the mean absolute difference between coulometric and pH-alkalinity determinations of TCO2on 22 parallel samples was 6.7 μmol kg−1or 0.5% of the mean. Community metabolism studies gave maximum rates of net production (TCO2uptake) and respiration (TCO2production) of 0.9-1.2 and 0.3-0.5 μmol l−1b−1, respectively. The results of this study provide solutions to the problems observed with the earlier manual prototype.