Pyrosequencing of the bacteria associated with Platygyra carnosus corals with skeletal growth anomalies reveals differences in bacterial community composition in apparently healthy and diseased tissues.
Pyrosequencing of the bacteria associated with Platygyra carnosus corals with skeletal growth anomalies reveals differences in bacterial community composition in apparently healthy and diseased tissues.
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DOI：
10.3389/fmicb.2015.01142
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发表时间：
2015
影响因子：
5.2
通讯作者：
Chiu JM
中科院分区：
文献类型：
--
作者：
Ng JC;Chan Y;Tun HM;Leung FC;Shin PK;Chiu JM
由于珊瑚疾病，全球珊瑚数量正在迅速减少。骨骼生长异常(SGA)或“珊瑚肿瘤”是一组影响全球珊瑚礁的珊瑚疾病，包括印度-太平洋地区的香港水域。为了更好地了解SGA对珊瑚中细菌群落的影响，我们首次使用16S核糖体rRNA基因焦磷酸测序技术研究了与SGA影响的肉骨扁平鱼表面健康和患病组织相关的细菌组成。分类分析表明，在表面健康的和患病的组织中，变形杆菌、拟杆菌、蓝藻和放线菌是主要的门。在OTU水平上，两种条件下的细菌群落组成有显著差异。病变组织中酸性细菌和双生单胞菌的丰度较高，而螺旋藻的丰度较低。红杆菌科、根瘤菌科、伽玛变形杆菌和胞噬-黄杆菌-类杆菌(CFB)的几种OTU与疾病组织有很强的相关性。这些细菌群可能包含与SGA的发展有关的潜在病原体，或者在损害健康的珊瑚宿主上繁殖的机会性第二或第三殖民者。我们建议在接种实验和科赫假设测试的基础上对这些细菌群进行进一步研究，以努力了解SGA的病因和进展。
Corals are rapidly declining globally due to coral diseases. Skeletal growth anomalies (SGA) or “coral tumors” are a group of coral diseases that affect coral reefs worldwide, including Hong Kong waters in the Indo-Pacific region. To better understand how bacterial communities may vary in corals with SGA, for the first time, we examined the bacterial composition associated with the apparently healthy and the diseased tissues of SGA-affected Platgyra carnosus using 16S ribosomal rRNA gene pyrosequencing. Taxonomic analysis revealed Proteobacteria, Bacteroidetes, Cyanobacteria, and Actinobacteria as the main phyla in both the apparently healthy and the diseased tissues. A significant difference in the bacterial community composition was observed between the two conditions at the OTU level. Diseased tissues were associated with higher abundances of Acidobacteria and Gemmatimonadetes, and a lower abundance of Spirochaetes. Several OTUs belonging to Rhodobacteraceae, Rhizobiales, Gammaproteobacteria, and Cytophaga-Flavobacterium-Bacteroidetes (CFB) were strongly associated with the diseased tissues. These groups of bacteria may contain potential pathogens involved with the development of SGA or opportunistic secondary or tertiary colonizers that proliferated upon the health-compromised coral host. We suggest that these bacterial groups to be further studied based on inoculation experiments and testing of Koch's postulates in efforts to understand the etiology and progression of SGA.