ROLE OF THE CHAPERONE PROTEIN HSP104 IN PROPAGATION OF THE YEAST PRION-LIKE FACTOR [PSI(+)]
ROLE OF THE CHAPERONE PROTEIN HSP104 IN PROPAGATION OF THE YEAST PRION-LIKE FACTOR [PSI(+)]
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DOI：
10.1126/science.7754373
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发表时间：
1995-05-12
期刊：
影响因子：
56.9
通讯作者：
LIEBMAN, SW
中科院分区：
文献类型：
--
作者：
CHERNOFF, YO;LINDQUIST, SL;LIEBMAN, SW
酵母非孟德尔因子[psi（+）]被认为是一种类似于哺乳动物朊病毒的自我修饰蛋白。这里报道了[psi（+）]因子的增殖需要中等量的伴侣蛋白Hsp 104。Hsp104的过量产生或失活引起[psi（+）]的损失。这些结果表明，伴侣蛋白在朊病毒样现象中起作用，并且一定水平的伴侣蛋白表达可以治愈朊病毒细胞而不影响活力。这可能导致涉及伴侣蛋白量或活性改变的抗朊病毒治疗。
The yeast non-Mendelian factor [psi(+)] has been suggested to be a self-modified protein analogous to mammalian prions. Here it is reported that an intermediate amount of the chaperone protein Hsp104 was required for the propagation of the [psi(+)] factor. Overproduction or inactivation of Hsp104 caused the loss of [psi(+)]. These results suggest that chaperone proteins play a role in prion-like phenomena, and that a certain level of chaperone expression can cure cells of prions without affecting viability. This may lead to antiprion treatments that involve the alteration of chaperone amounts or activity.