Gene structure of the novel cytochrome P4501D1 genes in stickleback (Gasterosteus aculeatus) and medaka (Oryzias latipes)
Gene structure of the novel cytochrome P4501D1 genes in stickleback (Gasterosteus aculeatus) and medaka (Oryzias latipes)
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DOI：
10.1016/j.marenvres.2008.02.011
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发表时间：
2008-07-01
影响因子：
3.3
通讯作者：
Stegeman, John J.
中科院分区：
文献类型：
--
作者：
Goldstone, Jared V.;Stegeman, John J.
细胞色素p4501 （CYP1）家族随着CYP1B和CYP1C亚家族的加入而扩大。我们最近在斑马鱼中发现了一个新的CYP1亚家族，CYP1D，它有一个单基因CYP1D1。在这里，我们检查了在其他鱼类基因组中发现的序列，即棘鱼（Gasterosteus aculeatus）和medaka (Oryzias latipes)，以寻找鱼类CYPIDI基因之间的相似性。在这两个物种中，CYP1D1的全长氨基酸序列与CYPlAs的一致性平均约为43%，而在序列比对歧义被掩盖后，其一致性接近50%。CYPID1有7个外显子，其大小和位置与斑马鱼CYPIDI和CYPIA中的外显子相似。然而，在medaka和棘鱼中，内含子距离要小得多。在不同物种的CYPlDI中也有不同数量的假定的异种反应元件。棘鱼或medaka基因是否能被芳烃受体（AHR）激动剂诱导尚不清楚。(C) 2008 Elsevier Ltd版权所有。
The cytochrome P450 1 (CYP1) family has expanded with the addition of the CYP1B and CYP1C subfamilies. We recently identified a new CYP1 subfamily in zebrafish, CYP1D, with a single gene, CYP1D1. Here we examined sequences found in other fish genomes, i.e., stickleback (Gasterosteus aculeatus) and medaka (Oryzias latipes), for similarities among fish CYPIDI genes. The full-length deduced amino acid sequences for CYP1D1 in these two species averaged about 43% identity to the CYPlAs, but nearly 50% when sequence alignment ambiguities were masked. CYPID1 has seven exons, similar in size and position to the exons in CYPIDI and CYPIA in zebrafish. However, the intronic distances were substantially smaller in the medaka and stickleback. There also were differing numbers of putative xenobiotic response elements in the CYPlDI of the various species. Whether the stickleback or medaka genes are inducible by aryl hydrocarbon receptor (AHR) agonists is yet to be determined. (C) 2008 Elsevier Ltd. All rights reserved.