A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence.
A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence.
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发表时间：
1990-11
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通讯作者：
Raymond N. DuBois;Michael W. McLaneS;Kevin RyderQ;Lester F. Laull;D. Nathans
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作者：
Raymond N. DuBois;Michael W. McLaneS;Kevin RyderQ;Lester F. Laull;D. Nathans
生长因子可迅速诱导一组编码调节蛋白的基因转录，其中许多基因已通过克隆得到鉴定。在这里，我们报告了在小鼠3T3细胞中由生长因子和其他各种细胞外信号因子诱导的与基因相对应的cDNAs的分析。该基因编码一个富含脯氨酸、丝氨酸和甘氨酸的核蛋白，命名为Nup475，由319个氨基酸组成，包含两个重复的半胱氨酸和组氨酸序列(CX8CX5CX3H)，提示一个新的重金属结合区。在大肠杆菌中生产的Nup475结合锌。它的mRNA存在于许多小鼠组织和细胞系中，尤其是在肠道、胸腺、再生肝脏和受干扰素刺激的巨噬细胞系中。我们推测Nup475是一个具有新的锌指结构的调节蛋白。
Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3H) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by gamma-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.