The attachment to, and invasion of HeLa cells by Salmonella typhimurium: the contribution of mannose-sensitive and mannose-resistant haemagglutinating activities.
The attachment to, and invasion of HeLa cells by Salmonella typhimurium: the contribution of mannose-sensitive and mannose-resistant haemagglutinating activities.
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鼠伤寒沙门氏菌对 HeLa 细胞的附着和侵袭：甘露糖敏感和甘露糖抗性血凝活性的贡献。
DOI：
10.1099/00221287-127-2-361
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Richardson,LA
中科院分区：
文献类型：
--
作者：
Jones,GW;Richardson,LA
本文研究了鼠伤寒沙门氏菌培养物的血凝活性与细菌对HeLa细胞的粘附以及HeLa细胞对细菌的内化之间的关系。粘附不受α-甲基-d-甘露糖苷抑制（即粘附具有甘露糖耐药性），6种供试菌株中仅4种产生1型菌毛和相关的甘露糖敏感性血凝素（MSHA）。另外2株属于无菌毛的FIRN群。当在37 °C下生长时，所有六种菌株的培养物都含有甘露糖抗性血凝（MRHA）活性，但当在18 °C下生长时，仅一种流苏状菌株和一种非流苏状菌株的培养物具有这种活性。从在18 °C和37 °C下生长的培养物以及具有表型MRHA阴性/MSHA阳性或MRHA阳性/MSHA阴性的突变菌株的比较中，得出的结论是MRHA活性负责鲑藻附着于HeLa细胞。只有对甘露糖具有抗性的细菌粘附导致HeLa细胞对细菌的内化。
The association of the haemagglutinating activities ofSalmonella typhimuriumcultures with bacterial adhesion to HeLa cells, and the internalization of the bacteria by HeLa cells, was studied. Adhesion was not inhibited by α-methyl-d-mannoside (i.e. adhesion was mannoseresistant), and only four of the six strains tested produced type 1 fimbriae and the associated mannose-sensitive haemagglutinin (MSHA). The other two strains belonged to the non-fimbriate FIRN biogroup. Cultures of all six strains contained a mannose-resistant haemagglutinating (MRHA) activity when grown at 37 °C, but cultures of only one fimbriate and one non-fimbriate strain did so when grown at 18 °C. From the comparison of cultures grown at 18 °C and 37 °C, and of mutant strains with the phenotypes MRHA-negative/MSHA-positive, or MRHA-positive/MSHA-negative, it was concluded that the MRHA activity was responsible for the attachment of salmonellae to HeLa cells. Only bacterial adhesion that was resistant to mannose resulted in the internalization of the bacteria by the HeLa cells.