Elemental microanalysis of fibroblasts by a scanning proton microprobe and application to Menkes’ disease
Elemental microanalysis of fibroblasts by a scanning proton microprobe and application to Menkes’ disease
复制标题
通过扫描质子微探针对成纤维细胞进行元素微量分析及其在门克斯病中的应用
DOI：
10.1007/bf02950785
复制
发表时间：
1994
影响因子：
3.9
通讯作者：
G. Legge
中科院分区：
文献类型：
--
作者：
G. Allan;J. Camakaris;G. Legge
扫描质子微探针已用于单个成纤维细胞的元素微量分析。探针检查了正常成纤维细胞和门克斯病患者培养的成纤维细胞，门克斯病是一种 X 连锁遗传性疾病，已知与铜代谢缺陷有关。将细胞培养在薄的超洁净尼龙箔上并保留在该表面上以供分析。聚焦的高能质子束用于照射选定的单个细胞，元素信息来自 X 射线和背散射质子数据。扫描质子微探针对痕量重元素的敏感性使得该元素信息可用于识别单个细胞是正常的还是门克斯突变体。细胞识别基于对由铜与细胞中存在的每种常量元素的比率形成的数据集进行判别分析的应用。这种细胞识别方法为门克斯病的快速诊断提供了希望。
A scanning proton microprobe has been used for the elemental microanalysis of individual fibroblast cells. Both normal fibroblasts and fibroblasts cultured from patients with Menkes' disease, an X-linked genetic disorder known to be associated with defective copper metabolism, were examined by the probe. The cells were cultured on a thin ultra-clean nylon foil and retained on that surface for analysis. The focused high-energy proton beam was used to irradiate selected individual cells and elemental information was derived from X-ray and backscattered proton data. The sensitivity of the scanning proton microprobe to trace concentrations of heavy elements has allowed this elemental information to be used to identify individual cells as being either normal or a Menkes' mutant. The cell identification was based on the application of discriminate analysis to a data set formed from the ratios of copper to each of the macroelements present in the cell. This method of cell identification offers the promise of rapid diagnosis of Menkes' disease.