Diffusible Signal Factor-Mediated Quorum Sensing Plays a Central Role in Coordinating Gene Expression of Xanthomonas citri subsp citri
Diffusible Signal Factor-Mediated Quorum Sensing Plays a Central Role in Coordinating Gene Expression of Xanthomonas citri subsp citri
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DOI：
10.1094/mpmi-07-11-0184
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发表时间：
2012-02-01
影响因子：
3.5
通讯作者：
Wang, Nian
中科院分区：
文献类型：
--
作者：
Guo, Yinping;Zhang, Yanping;Wang, Nian
扩散信号因子（DSF）家族信号介导的群体感应（QS）已在许多革兰氏阴性菌中被鉴定。黄单胞菌的QS途径。由三个主要的QS组件组成：RpfF、RpfC和RpfG。rpfF基因编码推定的烯酰辅酶A水合酶，其催化信号分子的合成。RpfC和RpfG作为一个双组分系统，用于细胞外DSF家族信号的感知和转导。为了进一步表征柑橘黄单胞菌亚种的QS调控网络。citri，我们通过使用转录组分析研究了RpfF、RpfC和RpfG调节子。QS突变体（rpfF、rpfC和rpfG）的转录组与野生型菌株的转录组的比较揭示了由所有三个QS组分控制的核心基因组，这表明RpfC-RpfG双组分系统是X中DSF家族信号介导的QS的主要和保守的信号感知和转导系统。柑桔属柑橘。由RpfF单独控制的独特基因表明QS途径的复杂性和X中额外的感觉机制的参与。柑桔属柑橘。RpfC和RpfG分别控制的独特基因支持RpfC和RpfG在基因调控中发挥更广泛作用而不是DSF信号转导的可能性。
Diffusible signal factor (DSF) family signal-mediated quorum sensing (QS) has been identified in many gram-negative bacteria. This QS pathway of Xanthomonas spp. consists of three major QS components: RpfF, RpfC, and RpfG. The rpfF gene encodes a putative enoyl-CoA hydratase that catalyzes the synthesis of the signal molecule. RpfC and RpfG serve as a two-component system for the perception and transduction of the extracellular DSF family signals. In order to further characterize the QS regulatory network in Xanthomonas citri subsp. citri, we investigated the RpfF, RpfC, and RpfG regulons by using transcriptome analyses. Comparison of the transcriptomes of the QS mutants (rpfF, rpfC, and rpfG) with that of the wild-type strain revealed a core group of genes controlled by all three QS components, suggesting that the RpfC-RpfG two-component system is a major and conserved signal perception and transduction system for DSF family signal-mediated QS in X. citri subsp. citri. The unique genes controlled by RpfF alone indicate the complexity of the QS pathway and the involvement of additional sensory mechanisms in X. citri subsp. citri. The unique genes controlled by RpfC and RpfG, respectively, support the possibility that RpfC and RpfG play broader roles in gene regulation other than transduction of DSF signals.