Functional dysbiosis within dental plaque microbiota in cleft lip and palate patients
Functional dysbiosis within dental plaque microbiota in cleft lip and palate patients
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DOI：
10.1186/s40510-019-0265-1
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发表时间：
2019-03-25
影响因子：
4.8
通讯作者：
Moriyama, Keiji
中科院分区：
文献类型：
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作者：
Funahashi, Kenta;Shiba, Takahiko;Moriyama, Keiji
背景龋病是一种多菌性疾病，在唇腭裂患者中普遍存在，尽管他们的口腔卫生保持良好。菌群失调，即牙菌斑微生物群内的不平衡状态，可能导致这些患者的龋齿患病率。然而，很少有人知道生态失调如何发生和影响致龋性。为了发现生态失调的迹象，我们对六名CLP患者和四名对照的斑块微生物群进行了元转录组分析。方法从每份样本中提取细菌总RNA并测序。分别从16S rRNA和mRNA读数估计细菌组成和功能谱。mRNA读数进一步用于估计细菌组成。在基于rRNA和基于mRNA的细菌组成中列出的物种被鉴定为具有原位功能的可行分类群（VTiF），并且具有高mRNA与rRNA比率的VTiF被认为是转录活性的。一个网络，每组连接两个VTiF，如果他们的mRNA丰度呈正相关。ResultsThe细菌的组成和功能配置文件本身并没有提供显着的迹象，在CLP组的生态失调。然而，组特异性活性分类群被确定，包括链球菌和普雷沃菌属物种的CLP组。此外，网络结构是不同的组之间，放线菌约氏菌和几个物种在CLP组的活性类群，这是连接的基础上正相关与statistical significance.ConclusionsFunctional生态失调内的菌斑菌群观察到，如组之间的网络结构的差异，并可能与致龋性。观察到的功能性生态失调是CLP患者口腔中微生物群内的一种无形变化。这可能强调了保持良好的口腔卫生的重要性，唇腭裂畸形患者。
BackgroundDental caries is a polymicrobial disease and prevalent among cleft lip and palate (CLP) patients, although their oral hygiene is well maintained. Dysbiosis, the state of imbalance within the dental plaque microbiota, may cause caries prevalence among these patients. However, little is known about how dysbiosis occurs and affects cariogenicity. To find dysbiotic signs, here we conducted a metatranscriptomic analysis for the plaque microbiota in six CLP patients and four controls.MethodsTotal bacterial RNA was extracted from each sample and sequenced. Bacterial composition and functional profiles were estimated from 16S rRNA and mRNA reads, respectively. The mRNA reads were further used for estimating bacterial composition. Species listed in both rRNA-based and mRNA-based bacterial composition were identified as viable taxa with in situ function (VTiF), and the VTiF with a high mRNA-to-rRNA ratio were considered to be transcriptionally active. A network was constructed for each group by connecting two VTiF if their mRNA abundances were positively correlated.ResultsThe bacterial composition and functional profiles themselves did not provide remarkable signs of dysbiosis in the CLP group. However, the group-specific active taxa were identified, including streptococcal and Prevotella species in the CLP group. Moreover, the network structure was different between groups; Actinomyces johnsonii and several species in the CLP group were the active taxa, which were connected based on positive correlations with statistical significance.ConclusionsFunctional dysbiosis within the plaque microbiota was observed such as difference of the network structure between groups, and may be associated with cariogenicity. The observed functional dysbiosis was an invisible change within the microbiota in the oral cavity of CLP patients. This may emphasize the importance of maintaining good oral hygiene of the patients with cleft anomalies.