The Use of HRP in Decolorization of Reactive Dyes and Toxicological Evaluation of Their Products.
The Use of HRP in Decolorization of Reactive Dyes and Toxicological Evaluation of Their Products.
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DOI：
10.4061/2010/703824
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发表时间：
2011-01-20
期刊：
影响因子：
--
通讯作者：
Ferreira-Leitão VS
中科院分区：
文献类型：
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作者：
da Silva MR;de Sá LR;Russo C;Scio E;Ferreira-Leitão VS
本文研究了辣根过氧化物酶（HRP）对Drimarene Blue X-3LR（DMBLR）、Drimarene Blue X-BLN（DMBBLN）、Drimarene Rubinol X-3LR（DMR）和Drimarene Blue CL-R（RBBR）等染料的脱色作用。在含有120 mg·L−1的反应介质中单独测试染料，考虑以下参数：温度（20-45°C），H2 O2浓度（0-4.44 mmol·L−1）和反应时间（5分钟，1和24 h）。以下条件：在35°C、0.55 mmol·L-1、1 h条件下，DMBLR、DMBBLN、DMR和RBBR的脱色率分别为99%、77%、94%和97%。应该提到的是，仅5分钟的反应就足以获得对DMBLR和RBBR的96%的脱色。在DMBLR、DMR和RBBR的脱色反应之后，可以观察到卤虫死亡率的降低，并且DMBBLN产生的产物的毒性没有显著增加。
This work studied the potential use of horseradish peroxidase (HRP) in the decolorization of the following textile dyes: Drimarene Blue X-3LR (DMBLR), Drimarene Blue X-BLN (DMBBLN), Drimarene Rubinol X-3LR (DMR), and Drimarene Blue CL-R (RBBR). Dyes were individually tested in the reaction media containing 120 mg·L−1, considering the following parameters: temperature (20–45°C), H2O2 concentration (0–4.44 mmol·L−1), and reaction time (5 minutes, 1 and 24 h). The following conditions: 35°C, 0.55 mmol·L−1, and 1h, provided the best set of results of color removal for DMBLR (99%), DMBBLN (77%), DMR (94%), and RBBR (97%). It should be mentioned that only 5 minutes of reaction was enough to obtain 96% of decolorization for DMBLR and RBBR. After the decolorization reactions of DMBLR, DMR, and RBBR, it was possible to observe the reduction of Artemia salina mortality and the no significant increase in toxicity for the products generated from DMBBLN.