Strengths and limitations of the neurosphere culture system
Strengths and limitations of the neurosphere culture system
复制标题
DOI：
10.1385/mn:34:3:153
复制
发表时间：
2006-12-01
影响因子：
5.1
通讯作者：
Parmar, Malin
中科院分区：
文献类型：
--
作者：
Jensen, Josephine B.;Parmar, Malin
在称为神经球的神经干细胞的自由漂浮培养物的最初报道之后（1，2），出现了使用这种有前途的培养系统的广泛研究。从理论上讲，这是一个近乎完美的系统，可以大规模生产用于细胞替代疗法的神经细胞，并分析和表征神经干细胞。十多年后，经过对神经球培养系统的严格审查和充分的实验测试，很明显，培养系统有几个缺点，它的有用性在几个应用中受到限制。尽管如此，过去十年产生的大部分高质量研究也表明，在正确的情况下，为了适当的目的，神经球坚持了他们最初的承诺。本文讨论了神经球培养系统的优点和缺点，关于其三个主要应用：作为神经干细胞的测定，作为神经发生和神经发育的模型系统，以及用于移植目的的神经干细胞的扩增。
After the initial reports of free- floating cultures of neural stem cells termed neurospheres ( 1,2), a wide array of studies using this promising culture system emerged. In theory, this was a nearperfect system for large- scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than a decade later, after rigorous scrutiny and ample experimental testing of the neurosphere culture system, it has become apparent that the culture system suffers from several disadvantages, and its usefulness is limited for several applications. Nevertheless, the bulk of high- quality research produced over the last decade has also shown that under the right circumstances and for the appropriate purposes, neurospheres hold up to their initial promise. This article discusses the pros and cons of the neurosphere culture system regarding its three major applications: as an assay for neural stem cells, as a model system for neurogenesis and neural development, and for expansion of neural stem cells for transplantation purposes.