Cytokeratin expression in human oral gingival epithelial cells: in vitro regulation by titanium-based implant materials.
Cytokeratin expression in human oral gingival epithelial cells: in vitro regulation by titanium-based implant materials.
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人口腔牙龈上皮细胞中的细胞角蛋白表达：钛基植入材料的体外调节。
DOI：
10.1016/s0142-9612(98)00041-6
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发表时间：
1998
期刊：
影响因子：
14
通讯作者：
M. Lissac
中科院分区：
文献类型：
--
作者：
C. Lagneau;J. Farges;P. Exbrayat;M. Lissac
为了评估人口腔上皮细胞中的细胞角蛋白表达是否会受到牙科手术中使用的植入材料的影响，将传代的人口腔牙龈上皮细胞接种在商业纯钛（CP-Ti）或Ti6Al4V钛合金上。约 15 天后，两种基质均达到汇合。此时细胞形成，是一层由密集的多边形细胞组成的组织层，并具有上皮细胞典型的丝状细胞角蛋白网络。使用免疫化学和免疫印迹分析来检测单个CK多肽（CK7、8、13、18和19）的量在培养基质功能中的变化。结果显示，无论使用何种培养基质，CK8、CK18 和 CK19 的表达水平都没有改变。与商业纯钛相比，钛合金上培养的上皮细胞中 CK13 的表达降低。相反，Ti6Al4V 合金中的 CK7 水平高于商业纯钛。这项研究表明钛基种植材料可以不同程度地影响口腔牙龈上皮细胞的表型。
To evaluate whether cytokeratin expression in human oral epithelial cells could be influenced by implant materials used in dental surgery, passaged human oral gingival epithelial cells were seeded on commercially pure titanium (CP-Ti) or on Ti6Al4V titanium alloy. Confluence was achieved after about 15 days on both substrates. Cells formed at that time, an organized layer of densely packed polygonal cells, and harbored a filamentous cytokeratin network typical of epithelial cells. Immunochemistry and immunoblot analysis were used to detect modifications of the amount of individual CK polypeptides (CK7, 8, 13, 18 and 19) in function of the culture substrate. Results showed that the level of CK8, CK18 and CK19 expression was not altered whatever the culture substrate used. The expression of CK13 was reduced in epithelial cells cultured on the titanium alloy, as compared with commercially pure titanium. Conversely, the level of CK7 was higher on the Ti6Al4V alloy than on commercially pure titanium. This study suggests that titanium-based implant materials could influence differently the phenotype of oral gingival epithelial cells.