Tollip, a negative regulator of TLR-signalling, is encoded by twin genes in salmonid fish.
Tollip, a negative regulator of TLR-signalling, is encoded by twin genes in salmonid fish.
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DOI：
10.1016/j.fsi.2008.04.002
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发表时间：
2008-07
影响因子：
4.7
通讯作者：
A. Rebl;B. Høyheim;U. Fischer;B. Köllner;E. Siegl;H. Seyfert
中科院分区：
文献类型：
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作者：
A. Rebl;B. Høyheim;U. Fischer;B. Köllner;E. Siegl;H. Seyfert
The factor Tollip is known to dampen TLR2- and TLR4-mediated signalling in mammals. No negative regulator of the piscine TLR-signalling cascade has been described so far, albeit a sizable collection of factors contributing to this ancient pathogen-sensing system are known from fish to date. We identified two closely related Tollip-encoding genes in Atlantic salmon (Salmo salar) and the respective ortholog mRNA molecules in rainbow trout (Oncorhynchus mykiss). The salmonid Tollip genes are segmented into 6 exons, similar to the human orthologous gene. The protein-encoding sequences are homologous to >97% among the twin factors and also between the species. Both encoded proteins contain a C2 domain and an ubiquitin system component, which are also characteristic features of the mammalian Tollip factor. We analysed the expression of these genes in trout. Both Tollip-encoding genes are ubiquitously and also equally expressed, as indicated by similar mRNA concentrations of both factors in any one tissue. Tollip expression was found to be up-regulated by viral infection. Our data suggest that the Tollip genes were duplicated before salmon and trout were evolutionary separated. Moreover, pathways dampening the activity of the TLR-cascade may have been conserved from lower vertebrates to mammals since Tollip, as a respective key factor has been highly conserved from fish to human.
