Comparison of SERS pH probe responses after microencapsulation within hydrogel matrices.
Comparison of SERS pH probe responses after microencapsulation within hydrogel matrices.
复制标题
水凝胶基质中微囊化后的SERS pH探针反应的比较。
DOI：
10.1117/1.jbo.26.9.097001
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发表时间：
2021-09
影响因子：
3.5
通讯作者：
McShane M
中科院分区：
文献类型：
--
作者：
Kotturi D;Paterson S;McShane M
重要性：个性化医疗需要跟踪个人的代谢物水平随着时间的推移，以检测异常和评估身体对药物的反应。植入式传感器提供了持续监测特定代谢物水平的有效手段，前提是它们在长时间内准确、稳定且无害。目的：四种类型的水凝胶嵌入pH敏感传感器进行了评估，其准确性，灵敏度，可逆性，寿命，动态响应，并在静态与动态条件和长期储存的一致性。方法：拉曼光谱首先用于校准拉曼活性水凝胶传感器在静态pH环境中的pH敏感峰的强度。然后评估暴露于流动池内变化的pH条件的水凝胶的动态响应。最后，测定储存5个月后的静态pH响应。结果如下：所有四种类型的水凝胶都允许表面增强拉曼光谱（Sers）传感器对当地环境的pH值做出响应，而不会引入干扰信号，从而产生一致的校准曲线。当pH值变化时，凝胶中的探针缓慢达到稳态，需要几个小时，并且发现响应时间在水凝胶之间变化。只有一种类型，即聚（甲基丙烯酸2-羟乙酯）（pHEMA），持续了五个月，动态范围没有显着下降。结论：虽然所有的水凝胶似乎是可行的候选人作为生物相容性主机的Sers传感化学，pHEMA被发现是最功能稳定的长时间间隔测试。聚（乙二醇）水凝胶表现出对pH值变化的最快速反应。由于这两种凝胶类型是共价交联的，通常不会降解，因此它们都比海藻酸钠更适合用作植入物。
Significance: Personalized medicine requires the tracking of an individual’s metabolite levels over time to detect anomalies and evaluate the body’s response to medications. Implanted sensors offer effective means to continuously monitor specific metabolite levels, provided they are accurate, stable over long time periods, and do no harm. Aim: Four types of hydrogel embedded with pH-sensitive sensors were evaluated for their accuracy, sensitivity, reversibility, longevity, dynamic response, and consistency in static versus dynamic conditions and long-term storage. Approach: Raman spectroscopy was first used to calibrate the intensity of pH-sensitive peaks of the Raman-active hydrogel sensors in a static pH environment. The dynamic response was then assessed for hydrogels exposed to changing pH conditions within a flow cell. Finally, the static pH response after 5 months of storage was determined. Results: All four types of hydrogels allowed the surface-enhanced Raman spectroscopy (SERS) sensors to respond to the pH level of the local environment without introducing interfering signals, resulting in consistent calibration curves. When the pH level changed, the probes in the gels were slow to reach steady-state, requiring several hours, and response times were found to vary among hydrogels. Only one type, poly(2-hydroxyethyl methacrylate) (pHEMA), lasted five months without significant degradation of dynamic range. Conclusions: While all hydrogels appear to be viable candidates as biocompatible hosts for the SERS sensing chemistry, pHEMA was found to be most functionally stable over the long interval tested. Poly(ethylene glycol) hydrogels exhibit the most rapid response to changing pH. Since these two gel types are covalently cross-linked and do not generally degrade, they both offer advantages over sodium alginate for use as implants.